LMCD1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85986

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all LMCD1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QLPIYDQDPSRCRGLLENELKLMEEFVKQYKSEALGVGEVALPGQGGLPKEEGKQQEKPEGAETTAATTNGSLSDPSKEVEYVCELCKGAAPPDSPVVYSDRAGYNKQWHPTCFVCAKCSEPLVDLIYFWKDGA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LMCD1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: LMCD1 Antibody [NBP1-85986]

Immunocytochemistry/ Immunofluorescence: LMCD1 Antibody [NBP1-85986]

Immunocytochemistry/Immunofluorescence: LMCD1 Antibody [NBP1-85986] - Staining of human cell line A-431 shows localization to plasma membrane & cell junctions. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: LMCD1 Antibody [NBP1-85986]

Immunohistochemistry-Paraffin: LMCD1 Antibody [NBP1-85986]

Immunohistochemistry-Paraffin: LMCD1 Antibody [NBP1-85986] - Staining in human skeletal muscle and liver tissues using anti-LMCD1 antibody. Corresponding LMCD1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: LMCD1 Antibody [NBP1-85986]

Immunohistochemistry-Paraffin: LMCD1 Antibody [NBP1-85986]

Immunohistochemistry-Paraffin: LMCD1 Antibody [NBP1-85986] - Staining of human skeletal muscle shows high expression.
LMCD1 Antibody

Immunohistochemistry-Paraffin: LMCD1 Antibody [NBP1-85986] -

Immunohistochemistry-Paraffin: LMCD1 Antibody [NBP1-85986] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells and nuclear positivity in molecular layer cells.
LMCD1 Antibody

Immunohistochemistry-Paraffin: LMCD1 Antibody [NBP1-85986] -

Immunohistochemistry-Paraffin: LMCD1 Antibody [NBP1-85986] -Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
LMCD1 Antibody - BSA Free Immunohistochemistry-Paraffin: LMCD1 Antibody - BSA Free [NBP1-85986]

Immunohistochemistry-Paraffin: LMCD1 Antibody - BSA Free [NBP1-85986]

Staining of human liver shows no cytoplasmic positivity in hepatocytes as expected.
LMCD1 Antibody - BSA Free Western Blot: LMCD1 Antibody - BSA Free [NBP1-85986]

Western Blot: LMCD1 Antibody - BSA Free [NBP1-85986]

Analysis in human cell lines A-549 and HeLa using Anti-LMCD1 antibody. Corresponding LMCD1 RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.

Applications for LMCD1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LMCD1

The protein encoded by the LMCD1 gene contains a cysteine-rich domain in the N-terminal region and 2 LIM domains in the C-terminal region. It also has several potential phosphorylation and N-myristoylation sites and a single potential N-glycosylation site. The presence of LIM domains implies involvement in protein-protein interactions. Expression of this gene has been detected in most tissues, with highest expression in skeletal muscle. Transcript variants utilizing alternative polyA signals have been observed. [provided by RefSeq]

Alternate Names

dyxin, LIM and cysteine-rich domains 1, LIM and cysteine-rich domains protein 1

Gene Symbol

LMCD1

Additional LMCD1 Products

Product Documents for LMCD1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for LMCD1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LMCD1 Antibody - BSA Free

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Protocols

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FAQs

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