LMO7 Antibody

Novus Biologicals | Catalog # NB100-1360

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all LMO7 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence ESTCVSASLPRS corresponding to internal region according to AAD33924.1.

Specificity

Please note that the amino acid sequence of the immuning peptide differs one amino acid from the current NCBI accession numbers (NP_005349.3, NP_056667.2). This antibody is expected to recognise both reported isoforms (NP_005349.3; NP_056667.2).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for LMO7 Antibody

Western Blot: LMO7 Antibody [NB100-1360]

Western Blot: LMO7 Antibody [NB100-1360]

Western Blot: LMO7 Antibody [NB100-1360] - Staining of human lung lysate (RIPA buffer, 30 ug total protein). Antibody at 1 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Frozen: LMO7 Antibody [NB100-1360]

Immunohistochemistry-Frozen: LMO7 Antibody [NB100-1360]

Immunohistochemistry-Frozen: LMO7 Antibody [NB100-1360] - LMO7 antibody stained hair cells in cochlea cross-sections of mouse CD1 P1. Alexa Fluor 488 used as secondary antibody to LMO7 for visualization. Image submitted by a verified customer review.

Applications for LMO7 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.5 - 2 ug/mL
Application Notes
WB: Approx. 70-75 kDa band and approx. 30 kDa band observed in human lung lysates (predicted MW of 92 kDa band according to AAD33924.1) and is in aggrement with scientific literature (Putilina et al). A minor band of unknown identity was also consistently observed at approx. 50 kDa. LMO7 antibody validated for IHC-Frozen from a verified customer review.

Reviewed Applications

Read 1 review rated 4 using NB100-1360 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LMO7

This protein contains a calponin homology (CH) domain, a PDZ domain, and a LIM domain. An F-box (FBX) domain is present in alternative splice variants. Members of the LIM protein family carry the LIM domain, a unique cysteine-rich zinc-binding domain. Members of the FBX protein family are involved in protein-protein interactions. The encoded protein may be involved in protein-protein interactions. Multiple alternative splice variants have been described but their full-length sequences have not been determined.

Alternate Names

FBX20F-box only protein 20, KIAA0858FBXO20F-box protein Fbx20, LIM domain 7, LIM domain only 7, LIM domain only protein 7, LMO-7, LOMP, zinc-finger domain-containing protein

Entrez Gene IDs

4008 (Human)

Gene Symbol

LMO7

UniProt

AAD33924. (Human)

Additional LMO7 Products

Product Documents for LMO7 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for LMO7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for LMO7 Antibody (1)

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1 Customer Rating
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Customer Images

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  • LMO7 Antibody
    Name: Gianluca Sampieri
    Application: Immunohistochemistry-Frozen
    Sample Tested: Cochlea Cross Section Postnatal Day and Cochlea Cross-section P1
    Species: CD1 Mouse
    Verified Customer | Posted 12/18/2018
    LMO7 antibody stained hair cells in cochlea cross-sections of mouse CD1 P1. Alexa 488 used as secondary antibody to LMO7 for visualization.
    LMO7 Antibody NB100-1360

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Protocols

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