Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) - Azide and BSA Free

Novus Biologicals | Catalog # H00010765-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunodiffusion

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1G10

Format

Azide and BSA Free
View all Lysine (K)-specific Demethylase 5B/KDM5B Primary Antibodies »

Product Specifications

Immunogen

KDM5B (NP_006609, 231 a.a. ~ 319 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AKRMRAEAMNIKIEPEETTEARTHNLRRRMGCPTPKCENEKEMKSSIKQEPIERKDYIVENEKEKPKSRSKKATNAVDLYVCLLCGSGN

Specificity

JARID1B (1G10)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images

Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02]

Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02]

Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02] - KDM5B monoclonal antibody (M02), clone 1G10. Analysis of KDM5B expression in HeLa.
Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02]

Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02]

Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02] - Analysis of monoclonal antibody to KDM5B on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02] - Analysis of monoclonal antibody to KDM5B on formalin-fixed paraffin-embedded human colon. Antibody concentration 1 ug/ml.
Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02]

Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02]

Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02] - Analysis of JARID1B expression in Hela NE.
ELISA: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02]

ELISA: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02]

ELISA: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02] - Detection limit for recombinant GST tagged KDM5B is approximately 0.03ng/ml as a capture antibody.

Applications

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunohistochemistry-Paraffin

1:100 - 1:2000

Western Blot

1:100 - 1:2000

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Lysine (K)-specific Demethylase 5B/KDM5B

JARID1B (Jumonji AT-rich interactive domain 1B) is a member of the JARID 1 family of proteins that catalyze the demethylation of histones on lysine. The Jarid 1 family members include JARID1A, JARID1B, JARID1C and JARID1D. The members of this family possess a Jumonji C (JmjC) and Jumonji N (JmjN) domain as well as one ARID (AT-rich interactive domain) and three PHD-type zinc fingers. As histone demethylases, JARID 1 proteins function as transcriptional repressors. JARID1B expression is largely restricted to testis and may play a critical role in tumorigenesis as it has been found to be upregulated in prostate and breast cancer. Alternative names for JARID1B include retinoblastoma-binding protein 2 homolog 1, RBP2-H1, cancer/testis antigen 31, CT31, PLU-1, PUT1, and KDM5B.

Alternate Names

CT31, Jarid1B, KDM5B, Lysine (K)specific Demethylase 5B, PLU-1, PUT1, RBBP2H1

Gene Symbol

KDM5B

Additional Lysine (K)-specific Demethylase 5B/KDM5B Products

Product Documents

Certificate of Analysis

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Product Specific Notices

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

Citations for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) - Azide and BSA Free

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Protocols

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