MAD2L2 Antibody (7B6I2)

Novus Biologicals | Catalog # NBP3-16451

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7B6I2 expressed in HEK293
View all MAD2L2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human MAD2B/MAD2L2 (Q9UI95). FEITQPPLLSISSDSLLSHVEQLLRAFILKISVCDAVLDHNPPGCTFTVLVHTREAATRNMEKIQVIKDFPWILADEQDVHMHDPRLIPLKTMTSDILKMQ

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MAD2L2 Antibody (7B6I2)

Western Blot: MAD2L2 Antibody (7B6I2) [NBP3-16451]

Western Blot: MAD2L2 Antibody (7B6I2) [NBP3-16451]

Western Blot: MAD2L2 Antibody (7B6I2) [NBP3-16451] - Western blot analysis of extracts of various cell lines, using MAD2B/MAD2L2 Rabbit mAb (NBP3-16451) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: MAD2L2 Antibody (7B6I2) [NBP3-16451]

Immunocytochemistry/ Immunofluorescence: MAD2L2 Antibody (7B6I2) [NBP3-16451]

Immunocytochemistry/Immunofluorescence: MAD2L2 Antibody (7B6I2) [NBP3-16451] - Immunofluorescence analysis of NIH-3T3 cells using MAD2B/MAD2L2 Rabbit mAb (NBP3-16451) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: MAD2L2 Antibody (7B6I2) [NBP3-16451]

Immunohistochemistry-Paraffin: MAD2L2 Antibody (7B6I2) [NBP3-16451]

Immunohistochemistry-Paraffin: MAD2L2 Antibody (7B6I2) [NBP3-16451] - Immunohistochemistry of paraffin-embedded mouse brain using MAD2B/MAD2L2 Rabbit mAb (NBP3-16451) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence: MAD2L2 Antibody (7B6I2) [NBP3-16451]

Immunocytochemistry/ Immunofluorescence: MAD2L2 Antibody (7B6I2) [NBP3-16451]

Immunocytochemistry/Immunofluorescence: MAD2L2 Antibody (7B6I2) [NBP3-16451] - Immunofluorescence analysis of C6 cells using MAD2B/MAD2L2 Rabbit mAb (NBP3-16451) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: MAD2L2 Antibody (7B6I2) [NBP3-16451]

Immunohistochemistry-Paraffin: MAD2L2 Antibody (7B6I2) [NBP3-16451]

Immunohistochemistry-Paraffin: MAD2L2 Antibody (7B6I2) [NBP3-16451] - Immunohistochemistry of paraffin-embedded rat brain using MAD2B/MAD2L2 Rabbit mAb (NBP3-16451) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: MAD2L2 Antibody (7B6I2) [NBP3-16451]

Immunohistochemistry-Paraffin: MAD2L2 Antibody (7B6I2) [NBP3-16451]

Immunohistochemistry-Paraffin: MAD2L2 Antibody (7B6I2) [NBP3-16451] - Immunohistochemistry of paraffin-embedded human colon using MAD2B/MAD2L2 Rabbit mAb (NBP3-16451) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for MAD2L2 Antibody (7B6I2)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MAD2L2

MAD2L2 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L2 is a homolog of MAD2L1.

Alternate Names

hREV7, MAD2 mitotic arrest deficient-like 2 (yeast), MAD2BREV7 homolog, MAD2-like protein 2, Mitotic arrest deficient 2-like protein 2, mitotic arrest deficient homolog-like 2, mitotic spindle assembly checkpoint protein MAD2B, REV7yeast, homolog)-like 2

Gene Symbol

MAD2L2

Additional MAD2L2 Products

Product Documents for MAD2L2 Antibody (7B6I2)

Certificate of Analysis

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Product Specific Notices for MAD2L2 Antibody (7B6I2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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