MAD4 Antibody (OTI7F7)

Novus Biologicals | Catalog # NBP2-46060

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI7F7
View all MAD4 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human MXD4(NP_006445) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

23.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MAD4 Antibody (OTI7F7)

Western Blot: MAD4 Antibody (OTI7F7) [NBP2-46060]

Western Blot: MAD4 Antibody (OTI7F7) [NBP2-46060]

Western Blot: MAD4 Antibody (OTI7F7) [NBP2-46060] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY MXD4 cDNA for 48 hrs and lysed.Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiMXD4.(1:2000)
Immunocytochemistry/ Immunofluorescence: MAD4 Antibody (OTI7F7) [NBP2-46060]

Immunocytochemistry/ Immunofluorescence: MAD4 Antibody (OTI7F7) [NBP2-46060]

Immunocytochemistry/Immunofluorescence: MAD4 Antibody (OTI7F7) [NBP2-46060] - Human keratinocytes, PFA-fixed, permabilisation: PBS-Triton 0.1% 20 minutes at RT, primary antibody at 1:250 in PBS-2% BSA, secondary antiboy conjugated to Alexa Fluor 594. Red: MAD4 antibody, Blue: DAPI. Image from verified customer review.
Immunohistochemistry-Paraffin: MAD4 Antibody (OTI7F7) [NBP2-46060]

Immunohistochemistry-Paraffin: MAD4 Antibody (OTI7F7) [NBP2-46060]

Immunohistochemistry-Paraffin: MAD4 Antibody (OTI7F7) [NBP2-46060] - Human tonsil within the normal limits using anti-MXD4 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 C for 3 min, (1:150)
Immunohistochemistry-Paraffin: MAD4 Antibody (OTI7F7) [NBP2-46060]

Immunohistochemistry-Paraffin: MAD4 Antibody (OTI7F7) [NBP2-46060]

Immunohistochemistry-Paraffin: MAD4 Antibody (OTI7F7) [NBP2-46060] - Paraffin-embedded human Kidney tissue within the normal limits using antiMXD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 C for 3 min, (1:150)
Immunohistochemistry-Paraffin: MAD4 Antibody (OTI7F7) [NBP2-46060]

Immunohistochemistry-Paraffin: MAD4 Antibody (OTI7F7) [NBP2-46060]

Immunohistochemistry-Paraffin: MAD4 Antibody (OTI7F7) [NBP2-46060] - Human liver tissue using anti-MXD4 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 C for 3 min, (1:150)

Applications for MAD4 Antibody (OTI7F7)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Reviewed Applications

Read 1 review rated 4 using NBP2-46060 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MAD4

MAD4 is a member of the MAD gene family. The MAD genes encode basic helix loop helix leucine zipper proteins that heterodimerize with MAX protein, forming a transcriptional repression complex. The MAD proteins compete for MAX binding with MYC, which heterodimerizes with MAX forming a transcriptional activation complex. Studies in rodents suggest that the MAD genes are tumor suppressors and contribute to the regulation of cell growth in differentiating tissues.

Alternate Names

BHLHC12, bHLHc12Max-interacting transcriptional repressor MAD4, Class C basic helix-loop-helix protein 12, Mad4 homolog, MAD4Max-associated protein 4, MAX dimerization protein 4, Max dimerizer 4, MST149, MSTP149, MXD4 MAX dimerization protein 4

Gene Symbol

MXD4

Additional MAD4 Products

Product Documents for MAD4 Antibody (OTI7F7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MAD4 Antibody (OTI7F7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for MAD4 Antibody (OTI7F7) (1)

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  • MAD4 Antibody (OTI7F7)
    Name: Julien COUTIER
    Application: Immunocytochemistry
    Sample Tested: Human keratinocytes
    Species: Human
    Verified Customer | Posted 08/30/2018
    Red : MAD4 Blue : DAPI
    PFA-fixed cells, permabilisation step is PBS-Triton 0.1% 20 minutes at room temperature, primary antibody used at 1/250e in PBS-BSA 2%, secondary antiboy conjugated to Alexa594
    MAD4 Antibody (OTI7F7) NBP2-46060

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Protocols

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