MAD4 Antibody (OTI7F7) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-72567
![Western Blot: MAD4 Antibody (OTI7F7)Azide and BSA Free [NBP2-72567]](https://resources.rndsystems.com/images/products/MAD4-Antibody-OTI7F7-Azide-and-BSA-Free-Western-Blot-NBP2-72567-img0001.jpg "Western Blot: MAD4 Antibody (OTI7F7)Azide and BSA Free [NBP2-72567]")
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI7F7
Format
Azide and BSA Free
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Product Specifications
Immunogen
Full length human recombinant protein of human MXD4(NP_006445) produced in E.coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
23.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MAD4 Antibody (OTI7F7) - Azide and BSA Free
Western Blot: MAD4 Antibody (OTI7F7)Azide and BSA Free [NBP2-72567]
Western Blot: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY MXD4 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiMXD4.(1:2000)![Immunohistochemistry: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567]](https://resources.rndsystems.com/images/products/MAD4-Antibody-OTI7F7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72567-img0004.jpg "Immunohistochemistry: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567]")
Immunohistochemistry: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567]
Immunohistochemistry: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567] - Human tonsil within the normal limits using anti-MXD4 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 C for 3 min, (1:150)![Flow Cytometry: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567]](https://resources.rndsystems.com/images/products/MAD4-Antibody-OTI7F7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72567-img0002.jpg "Flow Cytometry: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567]")
Flow Cytometry: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567]
Flow Cytometry: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567] - Human keratinocytes, PFA-fixed, permeabilization: PBS-Triton 0.1% 20 minutes at RT, primary antibody at 1:250 in PBS-2% BSA, secondary antibody conjugated to Alexa Fluor 594. Red: MAD4 antibody, Blue: DAPI. Image from verified customer review.![Immunohistochemistry: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567]](https://resources.rndsystems.com/images/products/MAD4-Antibody-OTI7F7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72567-img0003.jpg "Immunohistochemistry: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567]")
Immunohistochemistry: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567]
Immunohistochemistry: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567] - Human liver tissue using anti-MXD4 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 C for 3 min, (1:150)![Immunohistochemistry: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567]](https://resources.rndsystems.com/images/products/MAD4-Antibody-OTI7F7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72567-img0005.jpg "Immunohistochemistry: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567]")
Immunohistochemistry: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567]
Immunohistochemistry: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567] - Paraffin-embedded human Kidney tissue within the normal limits using antiMXD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 C for 3 min, (1:150)Applications for MAD4 Antibody (OTI7F7) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:2000
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Calculators
Background: MAD4
Alternate Names
BHLHC12, bHLHc12Max-interacting transcriptional repressor MAD4, Class C basic helix-loop-helix protein 12, Mad4 homolog, MAD4Max-associated protein 4, MAX dimerization protein 4, Max dimerizer 4, MST149, MSTP149, MXD4 MAX dimerization protein 4
Gene Symbol
MXD4
Additional MAD4 Products
Product Documents for MAD4 Antibody (OTI7F7) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MAD4 Antibody (OTI7F7) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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