MAP7 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84853

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MAP7 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IAKGALTGGTEVSALPCTTNAPGNGKPVGSPHVVTSHQSKVTVESTPDLEKQPNENGVSVQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MAP7 Antibody - BSA Free

Western Blot: MAP7 Antibody [NBP1-84853]

Western Blot: MAP7 Antibody [NBP1-84853]

Western Blot: MAP7 Antibody [NBP1-84853] - Analysis in control (vector only transfected HEK293T lysate) and MAP7 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: MAP7 Antibody [NBP1-84853]

Immunocytochemistry/ Immunofluorescence: MAP7 Antibody [NBP1-84853]

Immunocytochemistry/Immunofluorescence: MAP7 Antibody [NBP1-84853] - Staining of human cell line A-431 shows localization to cytosol & microtubules. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853]

Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853]

Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853] - Staining of human lymph node.
Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853]

Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853]

Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853] - Staining of human colon shows high expression.
Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853]

Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853]

Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853] - Staining in human cerebral cortex and skeletal muscle tissues.. Corresponding MAP7 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853]

Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853]

Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853]

Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853]

Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853] - Staining of human skeletal muscle shows no positivity in myocytes.
Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853]

Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853]

Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853] - Staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.
Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853]

Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853]

Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853] - Staining of human cerebral cortex, kidney, lymph node and testis using Anti-MAP7 antibody NBP1-84853 (A) shows similar protein distribution across tissues to independent antibody NBP1-84852 (B).
Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853]

Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853]

Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853] - Staining of human testis.

Applications for MAP7 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAP7

The product of this gene is a microtubule-associated protein that is predominantly expressed in cells of epithelial origin. Microtubule-associated proteins are thought to be involved in microtubule dynamics, which is essential for cell polarization and differentiation. This protein has been shown to be able to stabilize microtubules, and may serve to modulate microtubule functions. Studies of the related mouse protein also suggested an essential role in microtubule function required for spermatogenesis.

Alternate Names

E-MAP-115dJ325F22.2 (microtubule-associated protein 7 (EMAP115, E-MAP-115)), ensconsin, Epithelial microtubule-associated protein of 115 kDa, MAP-7, microtubule-associated protein 7EMAP115

Gene Symbol

MAP7

Additional MAP7 Products

Product Documents for MAP7 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MAP7 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MAP7 Antibody - BSA Free

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Protocols

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FAQs

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