MBD2 Antibody (24GB460)

Novus Biologicals | Catalog # NBP3-51325

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB460
View all MBD2 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human MBD2 (Uniprot: Q9UBB5)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MBD2 Antibody (24GB460)

MBD2 Antibody (24GB460)

Immunocytochemistry/ Immunofluorescence: MBD2 Antibody (24GB460)[NBP3-51325] -

Immunocytochemical staining of HepG2 cells with MBD2 antibody (1:1,000). Nuclei were stained blue with DAPI; MBD2 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
MBD2 Antibody (24GB460)

Flow Cytometry: MBD2 Antibody (24GB460)[NBP3-51325] -

Flow cytometric analysis of MBD2expression in HepG2 cells using MBD2 antibody (1:2,000). Green, isotype control; red, MBD2.
MBD2 Antibody (24GB460)

Western Blot: MBD2 Antibody (24GB460)[NBP3-51325] -

Western blotting analysis using MBD2 antibody (1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.

Applications for MBD2 Antibody (24GB460)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MBD2

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. [provided by RefSeq]

Long Name

Methyl-CpG-binding domain protein 2

Alternate Names

Demethylase, DMTase

Gene Symbol

MBD2

Additional MBD2 Products

Product Documents for MBD2 Antibody (24GB460)

Certificate of Analysis

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Product Specific Notices for MBD2 Antibody (24GB460)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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