Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 012 expressed in HEK293

Format

Azide and BSA Free
View all MCAM/CD146 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human MCAM/CD146 extracellular domain (Accession#: NP_006491.2; Met 1-Gly 559) .

Specificity

No cross-reactivity in ELISA with: Human CD66aHuman CD166Human CD106Human BCAMHuman CD226

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for MCAM/CD146 Antibody (012) - Azide and BSA Free

Western Blot: MCAM/CD146 Antibody (012) [NBP2-89202]

Western Blot: MCAM/CD146 Antibody (012) [NBP2-89202]

Western Blot: MCAM/CD146 Antibody (012) [NBP2-89202] - Lane A: CD146 konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell LysateLysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:132 kDa Observed band size: kDa
Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (012) [NBP2-89202]

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (012) [NBP2-89202]

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (012) [NBP2-89202] - Staining of human kidney showing membrane staining of endothelial cells (red arrow) (1:200).
Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (012) [NBP2-89202]

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (012) [NBP2-89202]

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (012) [NBP2-89202] - Staining of human stomach showing membrane staining of endothelial cells (red arrow), epithelial cells (green arrow). The left panel: 1:200. The right panel: 1:1000.
Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (012) [NBP2-89202]

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (012) [NBP2-89202]

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (012) [NBP2-89202] - Staining of human lymphonode showing membrane staining of endothelial cells (red arrow), smooth muscle cells (black arrow) (1:200).
MCAM/CD146 Antibody (012) - Azide and BSA Free

Western Blot: MCAM/CD146 Antibody (012) [NBP2-89202] -

Western Blot: MCAM/CD146 Antibody (012) [NBP2-89202] - NBP2-89202 at 1:500 dilution Lane A: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:132 kDa Observed band size:140 kDa

Applications for MCAM/CD146 Antibody (012) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunohistochemistry-Paraffin

1:100-1:500

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MCAM/CD146

CD146 belongs to the immunoglobulin supergene family with five immunoglobulin like domains (V-V-C2-C2-C2), a transmembrane region and a 63 residue cytoplasmic tail. The protein is a membrane glycoprotein that functions as a Ca2+ independent cell adhesion molecule involved in heterophilic cell to cell interactions. CD146 has a molecular size of 130 kDa in its reduced form (118 kDa unreduced), and N linked glycosylation accounts for fifty percent of the apparent molecular weight. In some cells the molecule carries a sulfate 3 glucuronyl moiety. Expression of the molecule was shown in a relatively limited spectrum of normal human tissues (endothelium, smooth muscle, and subpopulations of activated T cells) and malignant neoplasm (melanoma and breast carcinoma). The lineage specific expression pattern of CD146 can be useful in the differential diagnosis of certain lesions including melanomas and various types of gestational trophoblastic lesions. CD146 expression can promote tumor progression in human melanoma, through enhanced interaction between melanoma cells and endothelial cells. However, in breast carcinoma, CD146 may act as a tumor suppressor. Over expression of CD146 in breast carcinoma cells results in a more cohesive cell growth and in the formation of smaller tumors in nude mice. During implantation and placentation, CD146 is expressed by the intermediate trophoblast in the placental site and binds to its putative receptor in uterine smooth muscle cells thus limiting trophoblastic invasion in the myometrium. Monoclonal antibody specific for CD146 is an important tool for the identification and isolation of cells expressing CD146.

Long Name

Melanoma Cell Adhesion Molecule

Alternate Names

CD146, L-Gicerin, MUC18

Gene Symbol

MCAM

Additional MCAM/CD146 Products

Product Documents for MCAM/CD146 Antibody (012) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for MCAM/CD146 Antibody (012) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for MCAM/CD146 Antibody (012) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review MCAM/CD146 Antibody (012) - Azide and BSA Free and earn rewards!

Have you used MCAM/CD146 Antibody (012) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies