MCAM/CD146 Antibody (23GB2705)

Novus Biologicals | Catalog # NBP3-51158

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB2705
View all MCAM/CD146 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human MCAM/CD146 (Uniprot: P43121)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MCAM/CD146 Antibody (23GB2705)

MCAM/CD146 Antibody (23GB2705)

Immunocytochemistry/ Immunofluorescence: MCAM/CD146 Antibody (23GB2705)[NBP3-51158] -

Immunocytochemical staining of HepG2 cells with MCAM/CD146 antibody (1:1,000). Nuclei were stained blue with DAPI; MCAM/CD146 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
MCAM/CD146 Antibody (23GB2705)

Flow Cytometry: MCAM/CD146 Antibody (23GB2705)[NBP3-51158] -

Validation of MCAM/CD146 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with MCAM/CD146 antibody (1:2,000) and analyzed using BD flow cytometer.
MCAM/CD146 Antibody (23GB2705)

Flow Cytometry: MCAM/CD146 Antibody (23GB2705)[NBP3-51158] -

Flow cytometric analysis of MCAM/CD146expression in HepG2 cells using MCAM/CD146 antibody (1:2,000). Green, isotype control; red, CD146.
MCAM/CD146 Antibody (23GB2705)

Western Blot: MCAM/CD146 Antibody (23GB2705)[NBP3-51158] -

Western blotting analysis using MCAM/CD146 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for MCAM/CD146 Antibody (23GB2705)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MCAM/CD146

CD146 belongs to the immunoglobulin supergene family with five immunoglobulin like domains (V-V-C2-C2-C2), a transmembrane region and a 63 residue cytoplasmic tail. The protein is a membrane glycoprotein that functions as a Ca2+ independent cell adhesion molecule involved in heterophilic cell to cell interactions. CD146 has a molecular size of 130 kDa in its reduced form (118 kDa unreduced), and N linked glycosylation accounts for fifty percent of the apparent molecular weight. In some cells the molecule carries a sulfate 3 glucuronyl moiety. Expression of the molecule was shown in a relatively limited spectrum of normal human tissues (endothelium, smooth muscle, and subpopulations of activated T cells) and malignant neoplasm (melanoma and breast carcinoma). The lineage specific expression pattern of CD146 can be useful in the differential diagnosis of certain lesions including melanomas and various types of gestational trophoblastic lesions. CD146 expression can promote tumor progression in human melanoma, through enhanced interaction between melanoma cells and endothelial cells. However, in breast carcinoma, CD146 may act as a tumor suppressor. Over expression of CD146 in breast carcinoma cells results in a more cohesive cell growth and in the formation of smaller tumors in nude mice. During implantation and placentation, CD146 is expressed by the intermediate trophoblast in the placental site and binds to its putative receptor in uterine smooth muscle cells thus limiting trophoblastic invasion in the myometrium. Monoclonal antibody specific for CD146 is an important tool for the identification and isolation of cells expressing CD146.

Long Name

Melanoma Cell Adhesion Molecule

Alternate Names

CD146, L-Gicerin, MUC18

Gene Symbol

MCAM

Additional MCAM/CD146 Products

Product Documents for MCAM/CD146 Antibody (23GB2705)

Certificate of Analysis

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Product Specific Notices for MCAM/CD146 Antibody (23GB2705)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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