MCM5 Antibody (24GB4965)

Novus Biologicals | Catalog # NBP3-51696

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB4965
View all MCM5 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human MCM5 (Uniprot: P33992)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MCM5 Antibody (24GB4965)

MCM5 Antibody (24GB4965)

Immunocytochemistry/ Immunofluorescence: MCM5 Antibody (24GB4965)[NBP3-51696] -

Immunocytochemical staining of HepG2 cells with MCM5 antibody (1:1,000). Nuclei were stained blue with DAPI; MCM5 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
MCM5 Antibody (24GB4965)

Flow Cytometry: MCM5 Antibody (24GB4965)[NBP3-51696] -

Validation of MCM5 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with MCM5 antibody (1:2,000) and analyzed using BD flow cytometer.
MCM5 Antibody (24GB4965)

Flow Cytometry: MCM5 Antibody (24GB4965)[NBP3-51696] -

Flow cytometric analysis of MCM5expression in HepG2 cells using MCM5 antibody (1:2,000). Green, isotype control; red, MCM5.
MCM5 Antibody (24GB4965)

Western Blot: MCM5 Antibody (24GB4965)[NBP3-51696] -

Western blotting analysis using MCM5 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.
MCM5 Antibody (24GB4965)

Western Blot: MCM5 Antibody (24GB4965)[NBP3-51696] -

Western blotting analysis using MCM5 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit (minichromosome maintenance complex component 5.

Applications for MCM5 Antibody (24GB4965)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MCM5

MCM5 (Mini Chromosome Maintenance protein-5) is involved in the control of DNA replication. MCM proteins have DNA dependent ATPase motifs in their central domain which is conserved from yeast to mammals. MCM proteins form a complex which exhibits ATPase and helicase activities. In G0 phase levels of MCM 2 and 5 are lower than that of MCM7 and 3 but increase as the cell enters G1/S phase of the cell cycle. It has been reported that immunocytochemical assessment of Mcm5 expression may be of value in improving the accuracy of cervical smear testing for the detection of malignancy.

Alternate Names

CDC46 homolog, CDC46DNA replication licensing factor MCM5, EC 3.6.4.12, MCM5 minichromosome maintenance deficient 5, cell division cycle 46, MCM5 minichromosome maintenance deficient 5, cell division cycle 46 (S.cerevisiae), MGC5315, minichromosome maintenance complex component 5, minichromosome maintenance deficient (S. cerevisiae) 5 (cell division cycle 46), minichromosome maintenance deficient 5 (cell division cycle 46), P1-CDC46

Gene Symbol

MCM5

Additional MCM5 Products

Product Documents for MCM5 Antibody (24GB4965)

Certificate of Analysis

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Product Specific Notices for MCM5 Antibody (24GB4965)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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