MD-2 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56655

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MD2 Primary Antibodies »

Product Specifications

Immunogen

A full length recombinant protein was used as the immunogen.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit MD-2 Antibody - BSA Free (NB100-56655) is a polyclonal antibody validated for use in IHC and WB. Anti-MD-2 Antibody: Cited in 8 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for MD-2 Antibody - BSA Free

Western Blot: MD-2 Antibody [NB100-56655]

Western Blot: MD-2 Antibody [NB100-56655]

Western Blot: MD-2 Antibody [NB100-56655] - Lanes containing cell lysate from untreated cell are on the left (-) and lysates from IFNG treated cells are on the right (+). The proteins are identified by the corresponding gene names. The housekeeping enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was blotted as a control that is not altered by IFNG treatment. Except for GAPDH, the numbers under each gene name are the fold changes in mRNAs. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0185956) licensed under a CC-BY license. anti-TLR4 (Novus Biological NBP2-24538).
Western Blot: MD-2 Antibody [NB100-56655]

Western Blot: MD-2 Antibody [NB100-56655]

MD-2-Antibody-Western-Blot-NB100-56655-img0012.jpg
Immunohistochemistry-Paraffin: MD-2 Antibody [NB100-56655]

Immunohistochemistry-Paraffin: MD-2 Antibody [NB100-56655]

Immunohistochemistry-Paraffin: MD-2 Antibody [NB100-56655] - Analysis of MD2 in human brain tissue using an isotype control antibody (top left) and MD2 antibody (bottom left, right) at 10 ug/ml.
Western Blot: MD-2 Antibody [NB100-56655]

Western Blot: MD-2 Antibody [NB100-56655]

Western Blot: MD-2 Antibody [NB100-56655] - Image of anti-MD2. Total protein from human colon (lane 1) and human spleen (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-MD2 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Applications for MD-2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

5-10 ug/ml

Western Blot

0.5-2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MD2

MD2 is a secreted glycoprotein associating with the extracellular domain of Toll-like receptor 4 (TLR4) and trafficking to and from the cell surface and the Golgi apparatus. It has a signal peptide, two N terminal glycosylation sites, and seven Cys residues and consists of a heterogeneous collection of disulfide-linked oligomers. MD2 is an essential accessory glycoprotein required for LPS recognition and associates with toll-like receptor 4 on the cell surface to confer responsiveness to lipopolysaccyaride (LPS), and hence provides a link between the receptor and LPS signaling. It can also function as a soluble receptor for cells that do not otherwise express it. MD2 contains multiple trypsin cleavage sites and its expression is correlated inversely with trypsin activity.

Long Name

Myeloid Differentiation Protein 2/Lymphocyte Antigen 96

Alternate Names

ESOP-1, LY96, MD-2

Entrez Gene IDs

23643 (Human); 17087 (Mouse); 448830 (Rat)

Gene Symbol

LY96

UniProt

NP_056179 (Human)

Additional MD2 Products

Product Documents for MD-2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MD-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Monocyte Markers
Toll-Like Receptors

Citations for MD-2 Antibody - BSA Free

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Protocols

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