MEKK4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-35705

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MEKK4 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MEKK4 (NP_005913.2).

Sequence:
MREAAAALVPPPAFAVTPAAAMEEPPPPPPPPPPPPEPETESEPECCLAARQEGTLGDSACKSPESDLEDFSDETNTENLYGTSPPSTPRQMKRMSTKHQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

182 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MEKK4 Antibody - BSA Free

MEKK4 Antibody

Immunohistochemistry: MEKK4 Antibody [NBP3-35705] -

Immunohistochemistry: MEKK4 Antibody [NBP3-35705] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using MEKK4 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
MEKK4 Antibody

Western Blot: MEKK4 Antibody [NBP3-35705] -

Western Blot: MEKK4 Antibody [NBP3-35705] - Western blot analysis of various lysates using MEKK4 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
MEKK4 Antibody

Immunohistochemistry: MEKK4 Antibody [NBP3-35705] -

Immunohistochemistry: MEKK4 Antibody [NBP3-35705] - Immunohistochemistry analysis of paraffin-embedded Human placenta using MEKK4 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
MEKK4 Antibody

Immunohistochemistry: MEKK4 Antibody [NBP3-35705] -

Immunohistochemistry: MEKK4 Antibody [NBP3-35705] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using MEKK4 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Applications for MEKK4 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MEKK4

MEK kinase 4 (MEKK4), also known as MTK1, is a Ser/Thr protein kinase belonging to the MAP kinase kinase kinase family (1). This protein contains a protein kinase catalytic domain at the C-terminus. The N-terminal, non-kinase domain may contain a regulatory domain. MEKK4 regulates p38 and JNK, butnot ERK pathway, through the phosphorylation of the MAP2Ks MKK3 and MKK6 and MKK4 and MKK7 respectively (1-2).

Alternate Names

EC 2.7.11.25, FLJ42439, MAPKKK4yeast, homolog of, MEKK 4, mitogen-activated protein kinase kinase kinase 4

Gene Symbol

MAP3K4

Additional MEKK4 Products

Product Documents for MEKK4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MEKK4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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