MIF Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-81832
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Scientific Data Images for MIF Antibody - BSA Free
Western Blot: MIF Antibody [NBP1-81832]
MIF-Antibody-Western-Blot-NBP1-81832-img0020.jpgImmunohistochemistry-Paraffin: MIF Antibody [NBP1-81832]
Immunohistochemistry-Paraffin: MIF Antibody [NBP1-81832] - Staining of human skeletal muscle shows no positivity in myocytes as expected.Western Blot: MIF Antibody [NBP1-81832]
Western Blot: MIF Antibody [NBP1-81832] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.Immunohistochemistry-Paraffin: MIF Antibody [NBP1-81832]
Immunohistochemistry-Paraffin: MIF Antibody [NBP1-81832] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.Immunohistochemistry-Paraffin: MIF Antibody [NBP1-81832]
Immunohistochemistry-Paraffin: MIF Antibody [NBP1-81832] - Staining of human kidney moderate cytoplasmic positivity in cells in tubules.Immunohistochemistry-Paraffin: MIF Antibody [NBP1-81832]
Immunohistochemistry-Paraffin: MIF Antibody [NBP1-81832] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.Western Blot: MIF Antibody [NBP1-81832] -
Western Blot: MIF Antibody [NBP1-81832] - Expression & purification of MaMIF. (A) SDS-PAGE analysis of purified MaMIF suggesting an approximate molecular mass of ~12.5 kDa for the monomer. (B) Superdex 75 10/300 GL analytical size-exclusion chromatography profile of MaMIF suggesting an apparent molecular mass of ~39 kDa in solution, corresponding to the size of a trimeric form. (C) CD spectrum of MaMIF confirming a properly folded protein. The pie chart in the inset shows the secondary structure make-up of the protein. (D) Western blot analysis with polyclonal anti-His & anti-MIF antibodies confirming the expressed protein to be MaMIF. (E) Western blot analysis with isotype-matched primary antibody shows the background level of antibody binding to the protein. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31664114), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Immunocytochemistry/ Immunofluorescence: MIF Antibody [NBP1-81832]
Staining of human cell line U-251 MG shows localization to cytosol.Applications for MIF Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence
Immunohistochemistry
Immunohistochemistry-Paraffin
Western Blot
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Background: MIF
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Additional MIF Products
Product Documents for MIF Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for MIF Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars