MKL2 Antibody

Western Blot: MKL2 Antibody [NBP1-46209]

Western Blot: MKL2 Antibody [NBP1-46209] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MKL2 antibody NBP1-46209 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. MKL2 was also immunoprecipitated by rabbit anti-MKL2 antibody NBP1-46208, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

Immunohistochemistry-Paraffin: MKL2 Antibody [NBP1-46209]

Immunohistochemistry-Paraffin: MKL2 Antibody [NBP1-46209] - Section of human prostate carcinoma and linitis plastica stomach cancer. Antibody: Affinity purified rabbit anti-MKL2 used at a dilution of 1:200 (1ug/ml) and 1:1,000 (0.2ug/ml). Detection: DAB

Western Blot: MKL2 Antibody [NBP1-46209] -

Immunoblot analysis for ECM and fibrosis-related proteins and proteins involved in cholesterol biosynthesis. (A) Confirmatory analysis for protein expression changes in HTM cells subjected to CMS for 24 h. COL1A2, total laminin, MKL2, and PAI-1 showed a significant upregulation at 24 h of CMS (ST) compared to unstretched control (CTL). (B) Confirmatory analysis for proteins involved in cholesterol biosynthesis in HTM cells subjected to CMS for 24 h. HMGCS1 and n-SREBP2 showed a significant upregulation at 24 h of CMS (ST) compared to unstretched control (CTL). The results were based on immunoblot analysis with subsequent densitometric analysis. GAPDH was immunoblotted as the loading control. Circles and boxes in the histograms represent sample numbers for CTL and ST, respectively. Values represent mean +/- SEM, where n = 4 (biological replicates). *p ≤ 0.050 was considered significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36176278), licensed under a CC-BY license. Not internally tested by Novus Biologicals.