MLK3 Antibody (3D11) - Azide and BSA Free

Novus Biologicals | Catalog # H00004296-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3D11

Format

Azide and BSA Free
View all MLK3 Primary Antibodies »

Product Specifications

Immunogen

MAP3K11 (NP_002410, 741 a.a. ~ 847 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PPPGTSRSAPGTPGTPRSPPLGLISRPRPSPLRSRIDPWSFVSAGPRPSPLPSPQPAPRRAPWTLFPDSDPFWDSPPANPFQGGPQDCRAQTKDMGAQAPWVPEAGP

Specificity

MAP3K11 - mitogen-activated protein kinase kinase kinase 11 (3D11)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MLK3 Antibody (3D11) - Azide and BSA Free

Western Blot: MLK3 Antibody (3D11) [H00004296-M02]

Western Blot: MLK3 Antibody (3D11) [H00004296-M02]

Western Blot: MLK3 Antibody (3D11) [H00004296-M02] - Analysis of MAP3K11 expression in transfected 293T cell line by MAP3K11 monoclonal antibody (M02), clone 3D11.Lane 1: MAP3K11 transfected lysate(92.7 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: MLK3 Antibody (3D11) [H00004296-M02]

Immunohistochemistry-Paraffin: MLK3 Antibody (3D11) [H00004296-M02]

Immunohistochemistry-Paraffin: MLK3 Antibody (3D11) [H00004296-M02] - Analysis of monoclonal antibody to MAP3K11 on formalin-fixed paraffin-embedded human spleen. Antibody concentration 3 ug/ml.
Proximity Ligation Assay: MLK3 Antibody (3D11) [H00004296-M02]

Proximity Ligation Assay: MLK3 Antibody (3D11) [H00004296-M02]

Proximity Ligation Assay: MLK3 Antibody (3D11) [H00004296-M02] - Analysis of protein-protein interactions between CDC42 and MAP3K11. HeLa cells were stained with anti-CDC42 rabbit purified polyclonal 1:1200 and anti-MAP3K11 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
ELISA: MLK3 Antibody (3D11) [H00004296-M02]

ELISA: MLK3 Antibody (3D11) [H00004296-M02]

ELISA: MLK3 Antibody (3D11) [H00004296-M02] - Detection limit for recombinant GST tagged MAP3K11 is 0.1 ng/ml as a capture antibody.

Applications for MLK3 Antibody (3D11) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MLK3

The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. [provided by RefSeq]

Long Name

Mixed Lineage Kinase 3

Alternate Names

MAP3K11, PTK1, RHOE, SPRK

Entrez Gene IDs

4296 (Human)

Gene Symbol

MAP3K11

UniProt

NP_002410 (Human)

Additional MLK3 Products

Product Documents for MLK3 Antibody (3D11) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MLK3 Antibody (3D11) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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