Mouse Brorin/VWC2 Alexa Fluor® 405-conjugated Antibody

Catalog #: AF6730V Datasheet / COA / SDS

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AF6730V-100UG

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All Brorin/VWC2 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Brorin/VWC2 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Brorin/VWC2 in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant human Brorin/VWC2 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Brorin/VWC2
Ser28-Met324
Accession # Q8C8N3

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Brorin/VWC2

Mouse VWC2 (von Willebrand Factor C domain-containing protein 2; also Brorin and cradin) is a secreted, 35 kDa (predicted) member of the chordin family of proteins. It is expressed in the choroid plexus and by neurons in multiple sites, and serves to sequester BMP family proteins such as BMP-2 and BMP-6. BMP antagonism is suggested to generate neurons from neural precursor cells at the expense of astrocytes. Mouse VWC2 precursor is 324 amino acids (aa) in length. It contains a 27 aa signal sequence, one poly-Ala region (aa 124-129), and two von Willebrand factor C domains (aa 152-211 and 215-273) that likely serve as BMP interaction sites. Over aa 28-324, mouse VWC2 shares 90% aa identity with human VWC2.

Long Name

von Willebrand Factor A2 Domain

Entrez Gene IDs

375567 (Human); 319922 (Mouse); 501231 (Rat)

Alternate Names

Brain-specific chordin-like protein; Brain-Specific Chordin-Like; Brorin; PSST739; UNQ739; Von Willebrand Factor C Domain Containing 2; Von Willebrand Factor C Domain-Containing Protein 2; VWC2