Mouse CD11b/Integrin alpha M Antibody

Catalog #: MAB11241
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11241-100
MAB11241-SP

Save 15% on Select RUO Reagents. See Details.

Detection of Mouse CD11b/Integrin alpha M by Western Blot.

2 Images

Product Details

Citations (2)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD11b/Integrin alpha M Antibody Summary

Species Reactivity

Mouse

Specificity

Detects recombinant mouse CD11b/Integrin alpha M in Western blot.

Source

Monoclonal Rat IgG_2B Clone # 908649

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin alpha M beta 2
Phe17-Asn1105 (Integrin alpha M), Gln24-Asn702 (Integrin beta 2)
Accession # NP_032427 (Integrin alpha M) & P11835 (Integrin beta 2)

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Mouse CD11b/Integrin alpha M by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Mouse CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB11241) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CD11b/Integrin alpha M at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry

View Larger

Detection of CD11b/Integrin alpha M in RAW 264.7 Mouse Cell Line by Flow Cytometry RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB11241, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD11b/Integrin alpha M

The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha and a beta subunit. The Integrin alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/ beta 2, also known as MAC-1 and complement receptor type 3 (CR3). Integrin alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1, fibrinogen, and the C3 complement fragment, C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles (1,2).

References

Beller, D.J. et al. (1982) J. Exp. Med. 156:1000.
Ault, K.A. and T.A. Springer (1981) J. Immunol. 126:359.

Entrez Gene IDs

3684 (Human); 16409 (Mouse)

Alternate Names

antigen CD11b (p170); CD11 antigen-like family member B; CD11b antigen; CD11b; Cell surface glycoprotein MAC-1 subunit alpha; Complement component 3 receptor 3 subunit; CR-3 alpha chain; CR3A; CR3AMGC117044; Integrin alpha M; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; MO1A; neutrophil adherence receptor alpha-M subunit; Neutrophil adherence receptor; SLEB6

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse CD11b/Integrin alpha M Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

Depletion and activation of microglia impact metabolic connectivity of the mouse brain
Authors: J Gnörich, A Reifschnei, K Wind, A Zatcepin, ST Kunte, P Beumers, LM Bartos, T Wiedemann, M Grosch, X Xiang, MK Fard, F Ruch, G Werner, M Koehler, L Slemann, S Hummel, N Briel, T Blume, Y Shi, G Biechele, L Beyer, F Eckenweber, M Scheifele, P Bartenstei, NL Albert, J Herms, S Tahirovic, C Haass, A Capell, S Ziegler, M Brendel
Journal of Neuroinflammation, 2023-02-24;20(1):47.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Diet-dependent function of the extracellular matrix proteoglycan Lumican in obesity and glucose homeostasis
Authors: G Wolff, AE Taranko, I Meln, J Weinmann, T Sijmonsma, S Lerch, D Heide, AT Billeter, D Tews, D Krunic, P Fischer-Po, BP Müller-Sti, S Herzig, D Grimm, M Heikenwäld, WW Kao, A Vegiopoulo
Mol Metab, 2018-10-23;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot