Mouse CD38 Antibody

Detection of CD38 by Western Blot

Expression of CD38 by treatment with anti-PD-L1 antibody and anti-tumor effect of anti-CD38 antibody with anti-PD-L1 antibody in vivo. (A) Boxplots from bulk RNAseq analysis corresponding to the CD38 expression (B) Quantitative mRNA expression of CD38 by real-time RT-PCR analysis (C) Western blot showing expression of CD38 and actin (loading control)- in RPP (flank and orthotopic tumors) and RPM flank tumors, which were treated with vehicle or anti-PD-L1 antibody (300µg/body, once weekly). (D) Tumor growth curves (mean ± standard error [SE]) of IgG control, anti-PD-L1 antibody alone (200 µg/body, once weekly), anti-CD38 antibody (300 µg/body, once weekly), and anti-CD38 antibody plus anti-PD-L1 antibody groups in B6129F1 mice injected with RPP cells (SCLC cells with conditional loss of Trp53, p130, and Rb1; n = 6 in each group). P values were calculated by student-T test. Image collected and cropped by CiteAb from the following open publication (https://www.frontiersin.org/articles/10.3389/fimmu.2024.1348982/full), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of CD38 by Flow Cytometry

Change of expression of CD38 by chemotherapy and anti-tumor effect of anti-CD38 antibody with anti-PD-L1 antibody and chemotherapy. (A) CD38 expression on CD45+ total immune cell, CD4+ T cell, and CD8+ T cell in clinical specimen from the patients with SCLC. (B, C) Flow cytometry analysis was performed on H82, DMS114, and H187 cell lines treated with 0.3 or 3 µM cisplatin (upper) and 0.03 or 0.3 µM etoposide for 72 hours. (D) Tumor growth curves (mean ± standard error [SE]) of IgG control and vehicle, anti-PD-L1 antibody alone (200 µg/body, once weekly) and anti-CD38 antibody (300 µg/body, once weekly), cisplatin (CDDP) (2mg/kg) and etoposide (ETP) (3mg/kg), and anti-CD38 antibody, anti-PD-L1 antibody, CDDP and ETP groups in B6129F1 mice injected with RPP cells (SCLC cells with conditional loss of Trp53, p130, and Rb1; n = = 6 in each group). P values were calculated by Mann-Whiteney for (A), * p<0.0332 and student-T test for (D). Image collected and cropped by CiteAb from the following open publication (https://www.frontiersin.org/articles/10.3389/fimmu.2024.1348982/full), licensed under a CC-BY license. Not internally tested by R&D Systems.