CDCP1 (CUB-domain containing protein 1; also CD318 and SIMA135) is a novel, 135 kDa cell surface glycoprotein that is found on tumor, stem cells, keratinocytes and colonic epithelial cells. It is reported that this protein is overexpressed in colon and lung cancers. It is a type I transmembrane (TM) protein that is involved with cell adhesion. Mouse CDCP1 is synthesized as an 833 amino acid (aa) precursor. It contains an extracellular region with three CUB domains (aa 30‑667) and a phosphotyrosine site at Tyr731. When unligated, CDCP1 can be proteolytically cleaved between aa’s 270‑300. This generates an 80 kDa TM protein that may be missing the N-terminal CUB domain (aa 221‑350). Over aa’s 25‑667, mouse CDCP1 is 83% and 92% aa identical to human and rat CDCP1, respectively.
Key Product Details
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Product Specifications
Immunogen
Arg25-Ala667
Accession # Q5U462
Specificity
Clonality
Host
Isotype
Scientific Data Images for Mouse CDCP1 Antibody
CDCP1 in Mouse Embryo.
CDCP1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Mouse CDCP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4515) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (VC006). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing mouse brain. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Applications for Mouse CDCP1 Antibody
Immunohistochemistry
Sample: Immersion fixed frozen sections of mouse embryo (13 d.p.c.)
Western Blot
Sample: Recombinant Mouse CDCP1
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CDCP1
Long Name
Alternate Names
Gene Symbol
UniProt
Additional CDCP1 Products
Product Documents for Mouse CDCP1 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse CDCP1 Antibody
For research use only
Related Research Areas
Citations for Mouse CDCP1 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars