Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all CDCP1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CDCP1
Arg25-Ala667
Accession # Q5U462

Specificity

Detects mouse CDCP1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human CDCP1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse CDCP1 Antibody

CDCP1 in Mouse Embryo.

CDCP1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Mouse CDCP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4515) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (VC006). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing mouse brain. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Mouse CDCP1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (13 d.p.c.)

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CDCP1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CDCP1

CDCP1 (CUB-domain containing protein 1; also CD318 and SIMA135) is a novel, 135 kDa cell surface glycoprotein that is found on tumor, stem cells, keratinocytes and colonic epithelial cells. It is reported that this protein is overexpressed in colon and lung cancers. It is a type I transmembrane (TM) protein that is involved with cell adhesion. Mouse CDCP1 is synthesized as an 833 amino acid (aa) precursor. It contains an extracellular region with three CUB domains (aa 30‑667) and a phosphotyrosine site at Tyr731. When unligated, CDCP1 can be proteolytically cleaved between aa’s 270‑300. This generates an 80 kDa TM protein that may be missing the N-terminal CUB domain (aa 221‑350). Over aa’s 25‑667, mouse CDCP1 is 83% and 92% aa identical to human and rat CDCP1, respectively.

Long Name

CUB Domain Containing Protein 1

Alternate Names

CD318, SIMA135

Entrez Gene IDs

64866 (Human); 109332 (Mouse)

Gene Symbol

CDCP1

UniProt

Q5U462

Additional CDCP1 Products

Product Documents for Mouse CDCP1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CDCP1 Antibody

For research use only

Citations for Mouse CDCP1 Antibody

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Protocols

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