Mouse Collagen XIII alpha 1 Alexa Fluor® 350-conjugated Antibody

Catalog #: AF4627U Datasheet / COA / SDS

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AF4627U-100UG

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Mouse Collagen XIII alpha 1 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Collagen XIII alpha 1 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human (rh) COL13A1v4 is observed, and less than 1% cross-reactivity with rhCOL4A1, rhCOL25A1, rhCOL3A1, and rhCOL1A1 i

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Collagen XIII Î±1
Glu107-Gln565
Accession # AAH34164

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Collagen XIII alpha 1

Collagen XIII alpha 1 is an 85-95 kDa protein in the type 2 transmembrane collagen family (1). Mature mouse Collagen XIII alpha 1 consists of a 40 amino acid (aa) cytoplasmic domain, a 19 aa transmembrane segment, and a 692 aa extracellular domain (ECD). The ECD contains three collagenous regions separated by shorter non‑collagenous regions (2, 3). Within comparable regions of the ECD, mouse Collagen XIII alpha 1 shares 85% and 88% aa sequence identity with human and rat Collagen XIII alpha 1, respectively. Mouse Collagen XIII alpha 1 is extensively spliced, with some isoforms showing a tissue specific distribution (2, 4). Collagen XIII alpha 1 is widely expressed during development and in the adult (4, 5). It localizes to intercellular adherens junctions and cell-matrix focal adhesions (6, 7). Collagen XIII alpha 1 assembles into disulfide-linked trimers, a process that is enhanced by proline hydroxylation (2, 8). Trimerization involves triple helix formation within the collagenous domains, although portions of the non‑collagenous regions can also form coiled coils (8‑10). The ECD of trimeric Collagen XIII alpha 1 is an extended rod-like structure with two flexible hinges that correspond to non‑collagenous regions (11). Collagen XIII alpha 1 clusters in cholesterol-rich domains on the plasma membrane (2, 12), and it can be cleaved from the cell surface or intracellularly by a furin-like protease (12). Collagen XIII alpha 1 binds the extracellular matrix molecules fibronectin, heparin, integrin alpha 1, nidogen‑2, and perlecan (11, 13). The shed ECD retains its ability to bind fibronectin and can interfere with matrix formation (14).

Entrez Gene IDs

1305 (Human); 12817 (Mouse); 499431 (Rat)

Alternate Names

COL13A1; collagen alpha-1(XIII) chain; Collagen XIII alpha 1; collagen, type XIII, alpha 1; FLJ42485