Mouse F4/80 Antibody (521204)

R&D Systems | Catalog # MAB5580

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 521204
View all F4/80 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with mouse F4/80/EMR1
Gln28-Gly931
Accession # Q61549

Specificity

Detects mouse F4/80/EMR1.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse F4/80 Antibody (521204)

Detection of F4/80/EMR1 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry.

Detection of F4/80/EMR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse F4/80/EMR1 Monoclonal Antibody (Catalog # MAB5580, filled histogram) or isotype control antibody (MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113).

F4/80 in RAW 264.7 mouse monocyte/macrophage cell line.

F4/80 was detected in unfixed live cells fixed RAW 264.7 mouse monocyte/macrophage cell line using Rat Anti-Mouse F4/80 Monoclonal Antibody (Catalog # MAB5580) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Detection of F4/80/EMR1 by Immunohistochemistry

Detection of F4/80/EMR1 by Immunohistochemistry

Podoplanin deletion mitigates d-flow-induced subendothelial accumulation of platelets and monocytes/macrophages. (A, B) RAW264.7 cells were treated for 12 h by oscillatory shear (OS) following laminar shear (LS) treatment for 12 h. The expression of PDPN were examined by qRT-PCR (A) and flow cytometry (B). In (B), a representative half-shift histogram was shown in the left. N = 3 per group. Results are representative of 3 independent experiments. (C) The relative RNA expression level of PDPN in LCA treated with PCL for 2 days was evaluated using real-time RT-PCR with RCA as control. Each sample was collected from 4-5 mice. (D) Representative images of en face staining of PDPN expression from LCA after PCL for 2 days. Green, PDPN; red, F4/80; blue, CD31. Bar = 20 μm. White arrows indicate monocyte expressing PDPN. (E) Quantitative statistical analysis of PDPN fluorescence intensity. N ≥ 5 fields per group. (F) Co-localization analysis of monocytes/macrophages and PDPN by cellSens 3.1. The ordinate represents Pearson correlation coefficient (PCC; Rr), the abscissa represents the group. N = 16 fields from 3 mice per group. (G) The representative images of cross-section staining of LCA for platelet and monocyte/macrophage accumulation from Mye Pdpn-/- and WT littermate mice after PCL for 2 days. L, lumen. Green, CD42d; blue, F4/80; red, CD31. Bar = 20 μm. White arrows indicate platelets. (H, I) Quantitative analysis of CD42d+ (H) and F4/80+ (I) fluorescence area in the subendothelium. N ≥ 9 fields from 3 mice per group. subE: subendothelium. In all relevant panels, data are mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, by unpaired t-test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34815786), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of F4/80/EMR1 by Immunohistochemistry

Detection of F4/80/EMR1 by Immunohistochemistry

Effect of RES on HFpEF-induced inflammation. (A) Representative Ly6G and (B) F4/80 immunofluorescence staining. (C–E) Concentration of plasma pro-inflammatory cytokines IL-1 beta, IL-6, and TNF-alpha (n = 8 per group). (F–H) Results of IL-1 beta, IL-6, and TNF-alpha mRNA expressions (n = 8 per group). (I) Representative western blots and quantitative results of the IL-1 beta, IL-6, and TNF-alpha proteins (n = 8 per group). **p < 0.01 vs. sham-vehicle group. #p < 0.05 vs. HFpEF-vehicle group. ##p < 0.01 vs. HFpEF-vehicle group. Scale bars, 50 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34177571), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse F4/80 Antibody (521204)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed and unfixed live RAW 264.7 mouse monocyte/macrophage cell line

Reviewed Applications

Read 2 reviews rated 3 using MAB5580 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: F4/80

F4/80, also known as EMR1, is a 160 kDa extensively glycosylated protein in the EGF-TM7 family of adhesion molecules. It is primarily expressed on macrophages and dendritic cells in mouse but is restricted to eosinophils in human. F4/80 plays a role in the development of peripheral tolerance.

Alternate Names

DD7A5-7, Gpf480, Ly71

Entrez Gene IDs

13733 (Mouse); 316137 (Rat)

Gene Symbol

ADGRE1

UniProt

Q61549

Additional F4/80 Products

Product Documents for Mouse F4/80 Antibody (521204)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse F4/80 Antibody (521204)

For research use only

Citations for Mouse F4/80 Antibody (521204)

Customer Reviews for Mouse F4/80 Antibody (521204) (2)

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2 Customer Ratings
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Customer Images

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  • Mouse F4/80 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Liver
    Species: Mouse
    Verified Customer | Posted 06/05/2018
    Mouse F4/80 Antibody (521204) MAB5580
  • Mouse F4/80 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Colon tissue
    Species: Mouse
    Verified Customer | Posted 12/01/2017
    We tried 4 difference sources and none of them, including this one, works. Our experience is that no F4/80 antibody works for FFPE samples. Although it is not uncommon to see published positive staining, those data using FFPE samples cannot be trusted.
    Bio-Techne Response
    Technical service is following up

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Protocols

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FAQs for Mouse F4/80 Antibody (521204)

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  • Q: Does this F4/80/EMR1 antibody come in lyophilized form?

    A: Yes, we carry this F4/80 antibody in lyophilized form.

  • Q: What is the immunogen sequence of this F4/80/EMR1 antibodies antibody?

    A: A sequence corresponding to Gln28 - Gly931 of mouse F4/80/EMR1.

  • Q: Does this F4/80/EMR1 antibody come in lyophilized form?

    A: Yes, we carry this F4/80 antibody in lyophilized form.

  • Q: What is the immunogen sequence of this F4/80/EMR1 antibodies antibody?

    A: A sequence corresponding to Gln28 - Gly931 of mouse F4/80/EMR1.

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