Mouse Follistatin-related Gene Protein/FLRG Antibody

R&D Systems | Catalog # AF1255

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Follistatin-related Gene Protein/FLRG Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Follistatin-related Gene Protein/FLRG
Val24-Val256
Accession # Q9EQC7

Specificity

Detects mouse Follistatin-related Gene Protein/FLRG in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human FLRG is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse Follistatin-related Gene Protein/FLRG Antibody

Follistatin-related Gene Protein/FLRG Inhibition of Activin A-induced Hemoglobin Expression and Neutralization by Mouse Follistatin-related Gene Protein/FLRG Antibody.

Follistatin-related Gene Protein/FLRG Inhibition of Activin A-induced Hemoglobin Expression and Neutralization by Mouse Follistatin-related Gene Protein/FLRG Antibody.

Recombinant Mouse Follistatin-related Gene Protein/FLRG (Catalog # 1255-F3) inhibits Recombinant Human/Mouse/Rat Activin A (Catalog # 338-AC) induced hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase assay. Inhibition of Recombinant Human/Mouse/Rat Activin A (7.5 ng/mL) activity elicited by Recombinant Mouse Follistatin-related Gene Protein/FLRG (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Follistatin-related Gene Protein/FLRG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1255). The ND50 is typically 3-12 µg/mL.

Applications for Mouse Follistatin-related Gene Protein/FLRG Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse skin

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Follistatin-related Gene Protein/FLRG (Catalog # 1255-F3)

Neutralization

Measured by its ability to neutralize Follistatin‑related Gene Protein/FLRG inhibition of Activin A-dependent hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. The Neutralization Dose (ND50) is typically 3-12 µg/mL in the presence of 0.1 µg/mL Recombinant Mouse Follistatin‑related Gene Protein/FLRG and 7.5 ng/mL Recombinant Human/Mouse/Rat Activin A.

Reviewed Applications

Read 1 review rated 5 using AF1255 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Follistatin-related Gene Protein/FLRG

Follistatin-Related Gene Protein (FLRG), also known as follistatin-like 3 (FSTL3) is a glycoprotein belonging to the follistatin-module protein family. Mouse FLRG cDNA encodes a 256 amino acid (aa) residue protein with a putative 23 aa signal peptide, an N-terminal domain, two cysteine-rich follistatin-like domains (FS) and a C‑terminal acidic domain. Compared to follistatin, FLRG lacks the third FS domain found in follistatin. In addition, FLRG also lacks the heparin-binding domain found within the first amino-terminal FS domain of follistatin. Mouse and human FLRG share approximately 83% aa sequence homology. Like follistatin, FLRG has been shown to bind and inhibit the activities of TGF-beta family ligands including activin, BMP-2, -6, -7 and GDF-8/myostatin. While both FLRG and follistatin are located in a wide and overlapping range of adult and fetal tissue, their sites of peak expression differ: FLRG most highly in heart, lung, kidney, placenta and testis, while follistatin is highest in ovary and pituitary. The expression of FLRG is upregulated by TGF-beta and activin signaling through Smad proteins. Although FLRG is a secreted protein in many cell types, it has also been localized to the nuclear compartment in HeLa, 293 and CHO cells (1‑5).

References

  1. Tsuchida, K. et al. (2000) J. Biol. Chem. 275:40778.
  2. Sidis, Y. et al. (2002) Endocrinology 143:1613.
  3. Tortoriello, D.V. et al. (2001) Endocrinology 142:3426.
  4. Hill, J. et al. (2002) J. Biol. Chem. 277:40735.
  5. Bartholin, L. et al. (2001) Oncogene 20:5409.

Alternate Names

Follistatin-like 3, FSTL3

Entrez Gene IDs

10272 (Human); 83554 (Mouse)

Gene Symbol

FSTL3

UniProt

Q9EQC7

Additional Follistatin-related Gene Protein/FLRG Products

Product Documents for Mouse Follistatin-related Gene Protein/FLRG Antibody

Certificate of Analysis

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Product Specific Notices for Mouse Follistatin-related Gene Protein/FLRG Antibody

For research use only

Related Research Areas

Activin Family
BMP Family

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  • Mouse Follistatin-related Gene Protein/FLRG Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 07/08/2020
    Mouse Follistatin-related Gene Protein/FLRG Antibody AF1255

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