Mouse GLI-2 Alexa Fluor® 405-conjugated Antibody

Catalog #: AF3635V Datasheet / COA / SDS

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AF3635V-100UG

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Mouse GLI-2 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse GLI-2 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse GLI-2
Met1-Glu416
Accession # NP_001074594

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: GLI-2

Mouse GLI-2 is a 185 kDa biopotential transcription regulator of the hedgehog signaling pathway. It contains 5 conserved tandem C2H2 zinc finger domains that are flanked by a repression domain at the N-terminal region and an activation domain in the C-terminal region. At least two additional isoforms that have amino acid substitutions/truncations in the region corresponding to amino acid residues 192‑350 of isoform 1 have been described. Transcriptional activity of GLI-2 is regulated by proteolytic processing and degradation. Within the region used as immunogen, mouse GLI-2 shares 91% and 97% amino acid sequence homology with that of human and rat GLI-2, respectively.

Long Name

GLI-Kruppel family member GLI2

Entrez Gene IDs

2736 (Human); 14633 (Mouse); 304729 (Rat)

Alternate Names

GLI family zinc finger 2; GLI2; GLI-2; GLI-Kruppel family member GLI2; glioma-associated oncogene family zinc finger 2; HPE9tax helper protein 1; oncogene GLI2; tax helper protein 2; Tax helper protein; tax-responsive element-2 holding protein; tax-responsive element-25-bp sequence binding protein; THP; THP1; THP2; zinc finger protein GLI2