Mouse HSF1 Alexa Fluor® 488-conjugated Antibody

Catalog #: AF7130G Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7130G-100UG

Save 15% on Select RUO Reagents. See Details.

All HSF1 Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse HSF1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse HSF1 in direct ELISAs. In direct ELISAs, approximately 6% cross-reactivity with recombinant mouse HSF4 is observed and less than 1% cross-reactivity with recombinant human HSF2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse HSF1
Thr124-Tyr247
Accession # P38532

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: HSF1

HSF1 (Heat Shock Factor 1; also HSTF 1) is a 67-75 kDa member of the HSF family of proteins. It is constitutively expressed and functions as a transcriptional activator under heat shock conditions. Normally, it exists as an inactive, monomeric member of the HSP multichaperone complex. Following stress, it dissociates from the complex, homotrimerizes, undergoes multiple phosphorylations, and translocates to the nucleus. Nuclear HSF1 is SUMOylated on Lys298 and binds to heat shock elements in select genes such as HSP70. Mouse HSF1 is 525 amino acids (aa) in length. It contains a DNA binding domain (aa 15-120), two hydrophobic oligomerization regions (aa 130-203 and 380-405), a regulatory domain (aa 221-310), and a transactivation domain (aa 367-525). There are at least 13 potential Ser/Thr phosphorylation sites. When highly phosphorylated, HSF1 appears to run at about 82-85 kDa in SDS-PAGE; when SUMOylated, it runs anomalously at around 170 kDa in SDS-PAGE. One splice variant exists that shows a deletion of aa 413-434. Over aa 124-247, mouse HSF1 shares 94% and 99% aa identity with human and rat HSF1, respectively.

Long Name

Heat Shock Transcription Factor 1

Entrez Gene IDs

3297 (Human); 154991 (Mouse); 79245 (Rat)

Alternate Names

heat shock factor protein 1; heat shock transcription factor 1HSTF1HSF 1; HSF1; HSTF 1; HSTF1