Mouse HSF1 Alexa Fluor® 532-conjugated Antibody

Catalog #: FAB7130X Datasheet / COA / SDS

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FAB7130X-100UG

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Mouse HSF1 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse HSF1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human HSF2 or recombinant mouse HSF4 is observed.

Source

Monoclonal Rat IgG2a Clone # 744509

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant mouse HSF1
Thr124-Tyr247
Accession # P38532

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: HSF1

Heat Shock Factor 1 (HSF1) is a 75-80 kDa constitutively expressed protein that functions as a transcriptional regulator under heat shock conditions. Heat stress induces the serine hyperphosphorylation, sumoylation, trimerization, and translocation of HSF1 to the nucleus. Nuclear HSF1 binds to heat shock elements (HSE) and promotes the transcription of genes important in cell protection from heat stress. Mouse HSF1 contains a DNA binding domain (aa 16-120), two hydrophobic oligomerization motifs (aa 137-212 and aa 378-407), a regulatory domain (aa 221-310), and a transactivation domain (aa 367-525). Alternate splicing of mouse HSF1 generates a short isoform that lacks 22 amino acids of the transactivation domain. Within aa 124-247, mouse HSF1 shares 94% and 99% aa sequence identity with human and rat HSF1, respectively.

Long Name

Heat Shock Transcription Factor 1

Entrez Gene IDs

3297 (Human); 154991 (Mouse); 79245 (Rat)

Alternate Names

heat shock factor protein 1; heat shock transcription factor 1HSTF1HSF 1; HSF1; HSTF 1; HSTF1