Mouse IL-21 Antibody (149204)

R&D Systems | Catalog # MAB594

R&D Systems

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 149204
View all IL-21 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse IL‑21
Pro25-Ser146
Accession # Q9ES17

Specificity

Detects mouse IL-21 in Western blots. Does not cross-react with recombinant human IL‑21, recombinant mouse (rm) IL‑15, or rmIL‑12.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse IL-21 Antibody (149204)

Detection of IL-21 antibody in Activated Mouse Splenocytes antibody by Flow Cytometry.

Detection of IL‑21 in Activated Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were untreated (light orange filled histogram) or activated for 24 hours with 50 ng/mL PMA and 500 ng/mL Ca2+ionomycin (dark orange filled histogram) then stained with Mouse IL-21 Monoclonal Antibody (Catalog # MAB594) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Mouse IL-21 Antibody (149204)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: Mouse splenocytes treated with PMA and Ca2+ ionomycin, fixed with paraformaldehyde, and permeabilized with saponin

Western Blot

1 µg/mL
Sample: Recombinant Mouse IL‑21 (Catalog # 594-ML)

Flow Cytometry Panel Builder

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-21

Interleukin-21 (IL-21) and its receptor appear to play important roles in the regulation of the immune system. IL-21 is most related to IL-2, IL-4, and IL-15. IL-21 R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta and IL-4 R alpha. Mouse IL-21 is a 146 amino acid (aa) residue protein with a 24 aa signal peptide. Mouse and human IL-21 share 57% aa identity. IL‑21 is expressed by activated T cells. Although not fully elucidated, the IL-2 R gamma ( gamma c) chain appears to play a role in IL-21 R signaling. The IL-21/IL-21 R interaction appear to play important roles in B and T cell proliferation after antigen stimulation and NK cell maturation.

References

  1. Parrish-Novak, et al. (2000) Nature 408:57.
  2. Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
  3. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
  4. Asao, H. et al. (2001) J. Immunol. 167:1.

Long Name

Interleukin 21

Alternate Names

CVID11, IL21, Za11

Entrez Gene IDs

59067 (Human); 60505 (Mouse); 365769 (Rat); 442935 (Canine); 100344172 (Rabbit)

Gene Symbol

IL21

UniProt

Q9ES17

Additional IL-21 Products

Product Documents for Mouse IL-21 Antibody (149204)

Certificate of Analysis

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Product Specific Notices for Mouse IL-21 Antibody (149204)

For research use only

Citations for Mouse IL-21 Antibody (149204)

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