Interleukin 27 (IL-27) is a novel heterodimeric cytokine comprised of the IL-12 p35-related protein, p28, and the IL-12 p40-related protein, EBI-3 (Epstein‑Barr virus‑induced gene 3). IL-27 binds TCCR/WSX-1 on naïve CD4+ T cells and induces the expression of a functional IL-12 receptor, making these cells sensitive to IL‑12‑mediated Th1 cell development.
Mouse IL-27 p28/IL-30 Antibody
R&D Systems | Catalog # MAB1834
Key Product Details
Validated by
Biological Validation
Species Reactivity
Mouse
Applications
Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 234205
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Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse IL‑27
Tyr19-Pro228 (EBI3) & Phe29-Ser234 (p28)
Accession # O35228 (EBI3) & Q8K3I6 (p28)
Tyr19-Pro228 (EBI3) & Phe29-Ser234 (p28)
Accession # O35228 (EBI3) & Q8K3I6 (p28)
Specificity
Detects mouse IL-27 in Western blots. This antibody recognizes the p28 subunit either as part of a p28/EBI-3 heterodimer or as a free subunit.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse IL-27 p28/IL-30 Antibody
Detection of IL‑27 in LPS-treated RAW 264.7 Mouse Cell Line by Flow Cytometry.
RAW 264.7 mouse monocyte/macrophage cell line was unstimulated (light orange open histogram) or treated with 1 µg/mL LPS for 20 hours, then stained with Rat Anti-Mouse IL-27 p28/IL-30 Monoclonal Antibody (Catalog # MAB1834, dark orange filled histogram) or isotype control (Catalog # MAB0061, blue open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Applications for Mouse IL-27 p28/IL-30 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin
Sample: RAW 264.7 mouse monocyte/macrophage cell line treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin
Western Blot
1 µg/mL
Sample: Recombinant Mouse IL-27 p28/IL-30 (Catalog # 7430-ML)
Sample: Recombinant Mouse IL-27 p28/IL-30 (Catalog # 7430-ML)
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IL-27 p28/IL-30
Long Name
Interleukin 27 p28
Alternate Names
IL-30, IL27 p28
Gene Symbol
IL27
UniProt
Additional IL-27 p28/IL-30 Products
Product Documents for Mouse IL-27 p28/IL-30 Antibody
Product Specific Notices for Mouse IL-27 p28/IL-30 Antibody
For research use only
Citations for Mouse IL-27 p28/IL-30 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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