IL-5 is a disulfide-linked homodimeric cytokine that is secreted by T cells. IL-5 promotes the proliferation, differentiation, and activation of eosinophils. It binds to a receptor complex consisting of one IL-5-specific alpha chain and one non-binding common beta chain that is shared with the receptors for GM-CSF and IL-3.
Mouse IL-5 Antibody (TRFK4) [Biotin]
R&D Systems | Catalog # BAM705
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
ELISA Detection (Matched Antibody Pair)
Cited:
ELISA Development, ELISA Development (Detection), Luminex Development
Label
Biotin
Antibody Source
Monoclonal Rat IgG2A Clone # TRFK4
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Product Specifications
Immunogen
Mouse T cell clone-derived partially purified recombinant mouse IL-5
Specificity
Detects mouse IL-5 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human (rh) IL-5, recombinant mouse (rm) IL‑3, or rmGM-CSF is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse IL-5 Antibody (TRFK4) [Biotin]
Mouse IL-5 ELISA Standard Curve
Recombinant Mouse IL‑5 (Catalog # 405-ML) was serially diluted and captured by Rat Anti-Human/Mouse IL‑5 Monoclonal Antibody (Catalog # MAB405) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse IL‑5 Monoclonal Antibody (Catalog # MAB705) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Mouse IL-5 Antibody (TRFK4) [Biotin]
Mouse IL-5 Sandwich Immunoassay
ELISA Detection (Matched Antibody Pair)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IL-5
Long Name
Interleukin 5
Alternate Names
BCDF mu, BCGFII, EDF, Eo-CSF, IL5, TRF
Entrez Gene IDs
Gene Symbol
IL5
Additional IL-5 Products
Product Documents for Mouse IL-5 Antibody (TRFK4) [Biotin]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse IL-5 Antibody (TRFK4) [Biotin]
For research use only
Citations for Mouse IL-5 Antibody (TRFK4) [Biotin]
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