Mouse IL-6 Antibody (MP5-20F3)

R&D Systems | Catalog # MAB406

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Rat, Transgenic Mouse

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Neutralization, Immunocytochemistry, Bioassay, Blocking, ELISA Capture, ELISA Development, ELISA Development (Capture), In vivo assay, Functional Assay, Isotype Control, Luminex Development

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # MP5-20F3
View all IL-6 Primary Antibodies »

Product Specifications

Immunogen

COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant mouse IL-6

Specificity

Detects mouse IL-6 in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) IL‑6, recombinant porcine IL‑6, recombinant rat IL-6, rhIL-11, rhCT-1, or rhCLC is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse IL-6 Antibody (MP5-20F3)

Detection of Recombinant Mouse IL-6 antibody by Western Blot.

Detection of Recombinant Mouse IL‑6 by Western Blot.

Western blot shows 25 ng of Recombinant Mouse IL-6 (406-ML), Recombinant Human IL-6 (206-IL) and Recombinant Rat IL-6 (506-RL). PVDF Membrane was probed with 1 µg/mL of Rat Anti-Mouse IL-6 Monoclonal Antibody (Catalog # MAB406) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for IL-6 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Cell Proliferation Induced by IL‑6 and Neutralization by Mouse IL‑6 Antibody.

Cell Proliferation Induced by IL‑6 and Neutralization by Mouse IL‑6 Antibody.

Recombinant Mouse IL-6 (406-ML) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-6 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-6 Monoclonal Antibody (Catalog # MAB406). The ND50 is typically 0.005-0.025 µg/mL.
Detection of IL-6 by Western Blot

Detection of IL-6 by Western Blot

Role of ZFP36 in intestinal ischemia–reperfusion (I/R)-induced acute lung injury.C57BL/6 mice were subjected to 60 min of intestinal ischemia followed by 0, 30, 60, and 90 min of reperfusion as indicated. Sham mice were included as the control. A ZFP36 mRNA and protein expression in lung tissues were analyzed by RT-qPCR and Western blot (n = 6 per group, **P < 0.01). B Immunohistochemical staining of lung tissues for ZFP36 of sham and I/R 60 min. Scale bars: 50 μm. C–F Levels of IL-1 beta (C), TNF-alpha (D), and IL-6 (E) were measured by ELISA and Western blotting (F). G H&E staining of lung tissues. Red arrows outline collapsed alveoli, blue arrows outline multiple inflammatory cells infilitration, black arrows outline bronchial hemorrhage. Scale bars: 50 μm. H–J Arterial blood PaO2 (H), lung water content (I), and BALF protein content (J) were measured. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34238924), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of IL-6 by Immunocytochemistry/ Immunofluorescence

Detection of IL-6 by Immunocytochemistry/ Immunofluorescence

Increased expression of IL-6 in primary cultured osteoblasts and chondrocytes from MLII mice. (a) Signal log ratio (SLR) of differentially expressed genes (SLR ≥ 2 or ≤ -2) in terminally differentiated osteoblasts from wild-type (WT) and MLII mice related to the gene ontology (GO) "Bone biological processes": "Bone mineralization" (GO 0030282) and "Osteoclast differentiation" (GO 0030316). (b) IL-6 immunostaining (green) of WT and MLII osteoblasts. Nuclei were visualized by 4′,6-diamidino-2-phenylindole (DAPI) staining (blue). Scale bar: 10 μm. (c) Concentration of IL-6 in conditioned media from WT and MLII osteoblasts (n = 5, mean ± SD, ***p ≤ 0.001). (d) Expression levels of Il6 mRNA related to Gapdh in primary osteoblasts, chondrocytes and osteoclasts from WT and MLII mice (n = 3, mean ± SD, ***p ≤ 0.001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33574442), licensed under a CC-BY license. Not internally tested by R&D Systems.
Mouse IL-6 ELISA Standard Curve

Mouse IL-6 ELISA Standard Curve

Recombinant Mouse IL‑6 (Catalog # 406-ML) was serially diluted and captured by Rat Anti-Mouse IL‑6 Monoclonal Antibody (Catalog # MAB406) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-406-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Detection of IL-6 by Western Blot

Detection of IL-6 by Western Blot

Role of ZFP36 in intestinal ischemia–reperfusion (I/R)-induced acute lung injury.C57BL/6 mice were subjected to 60 min of intestinal ischemia followed by 0, 30, 60, and 90 min of reperfusion as indicated. Sham mice were included as the control. A ZFP36 mRNA and protein expression in lung tissues were analyzed by RT-qPCR and Western blot (n = 6 per group, **P < 0.01). B Immunohistochemical staining of lung tissues for ZFP36 of sham and I/R 60 min. Scale bars: 50 μm. C–F Levels of IL-1 beta (C), TNF-alpha (D), and IL-6 (E) were measured by ELISA and Western blotting (F). G H&E staining of lung tissues. Red arrows outline collapsed alveoli, blue arrows outline multiple inflammatory cells infilitration, black arrows outline bronchial hemorrhage. Scale bars: 50 μm. H–J Arterial blood PaO2 (H), lung water content (I), and BALF protein content (J) were measured. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34238924), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of IL-6 by Immunocytochemistry/ Immunofluorescence

Detection of IL-6 by Immunocytochemistry/ Immunofluorescence

Increased expression of IL-6 in primary cultured osteoblasts and chondrocytes from MLII mice. (a) Signal log ratio (SLR) of differentially expressed genes (SLR ≥ 2 or ≤ -2) in terminally differentiated osteoblasts from wild-type (WT) and MLII mice related to the gene ontology (GO) "Bone biological processes": "Bone mineralization" (GO 0030282) and "Osteoclast differentiation" (GO 0030316). (b) IL-6 immunostaining (green) of WT and MLII osteoblasts. Nuclei were visualized by 4′,6-diamidino-2-phenylindole (DAPI) staining (blue). Scale bar: 10 μm. (c) Concentration of IL-6 in conditioned media from WT and MLII osteoblasts (n = 5, mean ± SD, ***p ≤ 0.001). (d) Expression levels of Il6 mRNA related to Gapdh in primary osteoblasts, chondrocytes and osteoclasts from WT and MLII mice (n = 3, mean ± SD, ***p ≤ 0.001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33574442), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse IL-6 Antibody (MP5-20F3)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse IL‑6 (Catalog # 406-ML)

Neutralization

Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line. Nordan, R.P. et al. (1987) J. Immunol. 139:813. The Neutralization Dose (ND50) is typically 0.005-0.025 µg/mL in the presence of 0.25 ng/mL Recombinant Mouse IL‑6.

Mouse IL-6 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse IL‑6 Biotinylated Antibody (Catalog # BAF406)
  • Standard: Recombinant Mouse IL-6 Protein (Catalog # 406-ML)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 6 reviews rated 4.7 using MAB406 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-6

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature mouse IL-6 is 187 amino acids (aa) in length that is typically expressed as a 22-28 kDA molecular weight protein. Mouse IL-6 shares 39% and 85% aa sequence identity with human and rat IL-6, respectively. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor. IL-6, along with TNF-alpha and IL-1, function to drive the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise. In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells.

Long Name

Interleukin 6

Alternate Names

BSF-2, BSF2, IFNB2, IL6, MGI-2A

Entrez Gene IDs

3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)

Gene Symbol

IL6

Additional IL-6 Products

Product Documents for Mouse IL-6 Antibody (MP5-20F3)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IL-6 Antibody (MP5-20F3)

For research use only

Citations for Mouse IL-6 Antibody (MP5-20F3)

Customer Reviews for Mouse IL-6 Antibody (MP5-20F3) (6)

4.7 out of 5
6 Customer Ratings
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Showing  1 - 5 的 6 reviews Showing All
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  • IL-6 in mouse samples
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Lung tissue
    Verified Customer | Posted 06/03/2025
    IL-6 expression in WT animals
    1:20 in antibody diluent 2. Incubation time - 60min.
    Mouse IL-6 Antibody (MP5-20F3) MAB406
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.
  • Mouse IL-6 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Heart tissue
    Species: Mouse
    Verified Customer | Posted 08/12/2021
    Mouse IL-6 Antibody (MP5-20F3) MAB406
  • Mouse IL-6 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Heart tissue and Ear tissue
    Species: Mouse
    Verified Customer | Posted 02/10/2021
    Mouse IL-6 Antibody (MP5-20F3) MAB406
  • Name: Anonymous
    Application: Cell Invasion
    Sample Tested: Cancer-associated Fibroblasts and tumor cells
    Species: Mouse
    Verified Customer | Posted 04/11/2019
    Mouse IL-6 Antibody (MP5-20F3) MAB406
  • Name: Anonymous
    Application: Cell depletion
    Sample Tested: Pancreatic cancer cells
    Species: Mouse
    Verified Customer | Posted 05/11/2018
  • Mouse IL-6 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Human recombinant antibody
    Species: Mouse
    Verified Customer | Posted 12/08/2017
    Mouse IL-6 Antibody (MP5-20F3) MAB406

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