Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MAdCAM-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MAdCAM-1
Gln22-Thr365, Predicted
Accession # NP_038619

Specificity

Detects mouse MAdCAM-1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) MAdCAM-1, recombinant mouse (rm) ICAM-1, rmICAM-2, rmICAM-5, rmVCAM-1, and rhICAM-3.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse MAdCAM‑1 Antibody

Detection of Mouse MAdCAM-1 antibody by Western Blot.

Detection of Mouse MAdCAM‑1 by Western Blot.

Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse MAdCAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF993) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MAdCAM-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MAdCAM-1 antibody in Mouse Intestine by Immunohistochemistry (IHC-Fr).

MAdCAM‑1 in Mouse Intestine.

MAdCAM-1 was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse MAdCAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF993) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the endothelial cells of blood capillaries in intestinal villi. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse MAdCAM‑1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse intestine

Western Blot

0.25 µg/mL
Sample: NIH‑3T3 mouse embryonic fibroblast cell line

Reviewed Applications

Read 2 reviews rated 4 using AF993 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MAdCAM-1

Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) is an immunoglobulin (Ig) cell adhesion molecule family member. In addition to Ig domains, it contains a mucin-like domain and a membrane proximal domain with similarity to IgA. MAdCAM-1 is involved in lymphocyte homing to mucosal sites and is expressed on high endothelial venules (HEV) of both mesenteric lymph nodes and Peyer’s patches. It has also been found to be expressed on sinus-lining cells of the spleen. The integrin, alpha 4 beta 7, has been shown to function as the MAdCAM-1 receptor. The Ig domains of MAdCAM-1 have been found to be critical to alpha 4 beta 7 binding. The mucin domain has been shown to have activity in L-Selectin binding. MAdCAM-1 expression has been demonstrated to be up-regulated by TNF-alpha and IL-1 beta. MAdCAM-1 appears to play a role in inflammatory bowel disease (IBD) as its expression is highly up-regulated in IBD and most likely serves to recruit alpha 4 beta 7-expressing lymphocytes to the region. In vivo studies involving nonobese diabetic (NOD) mice have also suggested that MAdCAM-1/ alpha 4 beta 7 interaction plays a role in diabetes development in this model. Mouse MAdCAM-1 is a 405 amino acid (aa) residue protein with a 21 aa signal sequence, a 344 aa extracellular domain, a 20 aa transmembrane domain and a 20 aa cytoplasmic domain.

References

  1. Briskin, M.J. et al. (1993) Nature 363:461.
  2. Yang, X.D. et al. (1997) Diabetes 46:1542.
  3. Sampaio, S.O. et al. (1995) J. Immunol. 155:2477.
  4. Kraal, G. et al. (1995) Am. J. Pathol. 147:763.
  5. Berg, E.L. et al. (1993) Nature 366:695.
  6. Takeuchi, M. and V.R. Baichwal (1995) Proc. Natl. Acad. Sci. USA 92:3561.

Long Name

Mucosal Addressin Cell Adhesion Molecule 1

Alternate Names

MAdCAM1

Entrez Gene IDs

8174 (Human); 17123 (Mouse)

Gene Symbol

MADCAM1

UniProt

NP_038619

Additional MAdCAM-1 Products

Product Documents for Mouse MAdCAM‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse MAdCAM‑1 Antibody

For research use only

Citations for Mouse MAdCAM‑1 Antibody

Customer Reviews for Mouse MAdCAM‑1 Antibody (2)

4 out of 5
2 Customer Ratings
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50%
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  • Mouse MAdCAM-1 Antibody
    Name: Edward Sanderlin
    Application: Immunohistochemistry
    Sample Tested: Lymph node tissue and Colon tissue
    Species: Mouse
    Verified Customer | Posted 05/19/2016
    Tissues fixed in 10% Formalin and embedded in paraffin blocks. Antigen retrieval in Tris-Base EDTA buffer pH 9.0. Incubated overnight with MAdCAM-1 at a concentration of 5ug/ml. Used CTS008 (Anti-Goat HRP-DAB cell and tissue staining kit) and saw signal in endothelial cells with no background.
  • Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: See PMID: 23021255
    Species: Mouse
    Verified Customer | Posted 01/12/2015

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