Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Meprin beta Subunit/MEP1B Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Meprin beta Subunit/MEP1B
Leu21-Ser594 (Thr75Ile, Ile432Val)
Accession # Q61847

Specificity

Detects mouse Meprin beta Subunit/MEP1B in direct ELISAs and Western blots. In Western blots, approximately 40% cross-reactivity with recombinant human MEP1B is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Meprin  beta  Subunit/MEP1B Antibody

Detection of Mouse Meprin beta Subunit/MEP1B antibody by Western Blot.

Detection of Mouse Meprin beta Subunit/MEP1B by Western Blot.

Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Mouse Meprin beta Subunit/MEP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3300) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Meprin beta Subunit/MEP1B at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.
Meprin beta Subunit/MEP1B antibody in Mouse Intestine by Immunohistochemistry (IHC-Fr).

Meprin beta Subunit/MEP1B in Mouse Intestine.

Meprin beta Subunit/MEP1B was detected in perfusion fixed frozen sections of mouse intestine using 1 µg/mL Goat Anti-Mouse Meprin beta Subunit/MEP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3300) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the brush border of intestinal villi.View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse Meprin  beta  Subunit/MEP1B Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse intestine

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Meprin  beta  Subunit/MEP1B (Catalog # 3300-ZN), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Mouse small intestine tissue

Reviewed Applications

Read 1 review rated 5 using AF3300 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Meprin beta Subunit/MEP1B

Meprins are multimeric proteases composed of alpha and beta subunits, which are members of the astacin family of zinc endopeptidases (1, 2). Both subunits form disulfide-linked homo- or heterooligomers, which are also referred to as meprin A (composed of alpha subunits with or without beta subunits) and meprin B (composed of beta subunits only) (3). Although the two subunits share 42% identity in their amino acid sequence, they differ significantly in their oligomeric structure, post-translational processing and subsequently cellular location, and substrate and peptide bond specificity (4). The 704 amino acid sequence of mouse meprin beta subunit precursor consists of a signal peptide (residues 1-20), a pro region (residues 21-62), and a mature chain (residues 63-704) containing following domains, catalytic (residues 63-260), MAM (residues 261-430), MATH (residues 431-586), EGF-like (residues 607-647), transmembrane (residues 655-678), and cytoplasmic (residues 679-704). The pro enzyme terminating at residue 594 was expressed and the secreted protein purified from conditioned medium. The amino acid sequence has Ile and Val at position 75 and 432 instead of Thr and Ile, respectively. After trypsin treatment, the activated enzyme cleaved a flurogenic peptide, which contains Asp and Glu, the preferred residues found in the P1’ and P1 sites (3).

References

  1. Bond, J.S. and R.J. Beynon (1995) Protein Sci. 4:1247.
  2. Stocker, W. et al. (1995) Protein Sci. 4:823.
  3. Bertenshaw, G.P. et al. (2001) J. Biol. Chem. 276:13248.
  4. Ishmael, F.T. et al. (2005) J. Biol. Chem. 280:13895.

Alternate Names

MEP1B

Entrez Gene IDs

4225 (Human); 17288 (Mouse)

Gene Symbol

MEP1B

UniProt

Q61847

Additional Meprin beta Subunit/MEP1B Products

Product Documents for Mouse Meprin  beta  Subunit/MEP1B Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Meprin  beta  Subunit/MEP1B Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Mouse Meprin  beta  Subunit/MEP1B Antibody

Customer Reviews for Mouse Meprin  beta  Subunit/MEP1B Antibody (1)

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  • Mouse Meprin  beta  Subunit/MEP1B Antibody
    Name: Anonymous
    Application: Western blot and immunofluorescence
    Sample Tested: C57BL/6J urine and C57BL/6J kidney
    Species: Mouse
    Verified Customer | Posted 08/20/2019
    Western blot on mouse kidney tissue and mouse urine; immunofluorescence on kidney sections 1:200
    1:5000 for WB
    Mouse Meprin  beta  Subunit/MEP1B Antibody AF3300

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Protocols

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