Mouse MMP-7 Antibody

R&D Systems | Catalog # AF2967

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MMP-7 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MMP‑7
Leu18-Leu264
Accession # AAA99983

Specificity

Detects mouse MMP-7 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human MMP‑7 is observed, and less than 2% cross-reactivity with recombinant mouse (rm) MMP-2, rmMMP-3, rmMMP-8, and rmMMP-9 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse MMP-7 Antibody

MMP-7 antibody in Mouse Intestine by Immunohistochemistry (IHC-Fr).

MMP‑7 in Mouse Intestine.

MMP‑7 was detected in perfusion fixed frozen sections of mouse small intestine using Goat Anti-Mouse MMP‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2967) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of MMP-7 by Immunohistochemistry

Detection of MMP-7 by Immunohistochemistry

Deletion of Nkx2-1, Foxa1 and Foxa2 at initiation blocks gastric differentiation and induces expression of squamocolumnar junctional markers in lung neoplasia.(A) Correlation between differentially expressed genes identified in single-cell analysis (C1 vs. C2, X axis) and population analysis (KrasLSL-G12D/+(K) vs. KrasLSL-G12D/+; Nkx2-1F/F (KN), Y axis). (B) tSNE plot of single high-quality tumor cells (this study) and E18.5 type two pneumocytes (AT2) from murine lung (Treutlein et al.). Color indicates cluster. Shape indicates source of cell. (C) Principal component analysis (PCA) of cancer cell clusters (C1–C3) based on genes comprising invasive mucinous adenocarcinoma signature (Guo et al). (D) Hierarchical clustering on principal components (HCPC) of the three cancer cell clusters (C1–C3) and normal murine tissue. The first two dimensions of a principal component analysis (PCA) is overlayed with the hierarchical clustering of the first five PCA dimensions. Dot color indicates cluster to which sample was assigned in K-means clustering. Gray ovals highlight multiple samples from the same tissue. ‘Glandular’ indicates glandular stomach. (E) IHC for indicated proteins in lung neoplasia 5 weeks after initiation with PGK-Cre lentivirus in KrasLSL-G12D/+; Nkx2-1F/F; Foxa1F/F; Foxa2F/F mice and controls. Scale bar: 100 microns. (F) IHC for FoxA1 and FoxA2 at the squamocolumnar junction (SCJ) of glandular stomach and forestomach (left panels) and in proximal forestomach (right panels). Arrows point to SCJ. Scale bars: 100 microns. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30475207), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse MMP-7 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse small intestine

Reviewed Applications

Read 1 review rated 5 using AF2967 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MMP-7

Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-7 (matrilysin) is expressed in epithelial cells of normal and diseased tissues, and is capable of digesting a large series of proteins of the extracellular matrix including collagen IV and X, gelatin, casein, laminin, aggrecan, entactin, elastin and versican (1). MMP-7 is implicated in the activation of other proteinases such as plasminogen, MMP-1, MMP-2, and MMP-9. In addition to its roles in connective tissue remodeling and cancer, MMP-7 also regulates intestinal alpha ‑defensin activation in innate host defense, releases tumor necrosis factor-alpha in a model of herniated disc resorption, and cleaves FasL to generate a soluble form in a model of prostate involution. Structurally, MMP-7 is the smallest of the MMPs and consists of two domains: a pro-domain that is cleaved upon activation and a catalytic domain containing the zinc-binding site.

References

  1. Woessner, J.F. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 532.

Long Name

Matrix Metalloproteinase 7

Alternate Names

Matrilysin, MMP7

Entrez Gene IDs

4316 (Human); 17393 (Mouse)

Gene Symbol

MMP7

UniProt

AAA99983

Additional MMP-7 Products

Product Documents for Mouse MMP-7 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse MMP-7 Antibody

For research use only

Citations for Mouse MMP-7 Antibody

Customer Reviews for Mouse MMP-7 Antibody (1)

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  • Mouse MMP-7 Antibody
    Name: Eduardo Candelario-Jalil
    Application: Western Blot
    Sample Tested: Ileum and jejunum homogenates
    Species: Mouse
    Verified Customer | Posted 09/03/2020
    Mouse MMP-7 Antibody AF2967

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