Mouse MOG Antibody

MOG in Mouse Brain.

MOG was detected in perfusion fixed frozen sections of mouse brain (cortex) using 15 µg/mL Goat Anti-Mouse MOG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2439) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of MOG by Western Blot

Changes in myelin proteins and cholesterol metabolism related proteins in aged OPCs.A, Heat map showing the increased expression of myelin proteins in aged OPCs. Z score indicates the mean of six independent biological replicates of the proteomics dataset. Lower expression is represented in blue, and higher expression is shown in yellow. B, Representative Western blotting showing myelin protein expression in A2B5+ OPCs and MOG+ oligodendrocytes from different ages. C, Representative immunocytochemistry images of purified OPCs cultured for 6 days in vitro and immunostained for CLDN11 and MOG. D, Bar graphs indicating IHC quantification of myelin protein intensity per A2B5+ cells across the different age groups (each biological replicate is represented by a single dot) Scale bar 100 μm (n = 3, Mean ± S.E.M. shown). E, F, Heat maps showing the expression changes for proteins involved in cholesterol biosynthesis or cholesterol transport, respectively. Z score indicates the mean of six independent biological replicates and lower expression is shown in blue, and higher expression is shown in yellow. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32434922), licensed under a CC-BY license. Not internally tested by R&D Systems.