Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 463004
View all EGF Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse EGF
Trp29-Arg1029
Accession # P01132

Specificity

Detects mouse Pro EGF in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) Amphiregulin, rmEpigen, rmEpiregulin, rmEGF, rmMFG-E8, recombinant human (rh) EGF, rhHB-EGF, rhHRG alpha, rhHRG beta, or recombinant rat EGF is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Pro EGF Antibody

Pro EGF antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr).

Pro EGF in Mouse Kidney.

Pro Epidermal Growth Factor (Pro EGF) was detected in perfusion fixed frozen sections of mouse kidney using Rat Anti-Mouse Pro EGF Monoclonal Antibody (Catalog # MAB5256) at 10 µg/mL for 3 hours at room temperature. Tissue was stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Mouse Pro EGF Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse kidney

Western Blot

1 µg/mL
Sample: Recombinant Mouse Pro EGF aa 29-1029 (Catalog # 4095-EG) under non-reducing conditions only

Reviewed Applications

Read 2 reviews rated 5 using MAB5256 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EGF

EGF is the prototypic member of a family of growth factors that also includes amphiregulin, betacellulin, epigen, epiregulin, HB-EGF, neuregulins-1 through -6, and TGF-alpha (1). These proteins contain EGF-like domains with three intramolecular disulfide bonds between conserved cysteines (2). EGF family members are synthesized as transmembrane preproproteins with varying numbers of EGF-like domains (3). The extracellular region of mouse pro-EGF contains eight LDL R class B repeats and nine EGF-like domains (4). Mature EGF is derived from the juxtamembrane EGF-like domain. EGF binds ErbB1 and induces the formation of homodimers or heterodimers containing ErbB2 (5). Pro-EGF is most highly expressed in the submaxillary gland and kidney (6). In the kidney, the 190 kDa preproprotein is cleaved by membrane-associated serine proteases, liberating the extracellular region which is subsequently processed into smaller fragments including the 6 kDa mature EGF (7‑10). The various cleavage products produced in the kidney also are present in urine (9‑11). In the submaxillary gland, however, nearly all EGF is processed intracellularly and stored in secretory vesicles (6, 12). The soluble precursor binds ErbB1 and induces cellular proliferation, although it is significantly less potent than mature EGF (8, 9). In human thyroid carcinoma cells, a splice variant of pro-EGF with a deletion in the cytoplasmic domain induces increased proliferative activity relative to wild-type pro-EGF (13). Within the extracellular region, mouse pro-EGF shares 79% amino acid sequence identity with rat pro-EGF and 67%‑69% with human, canine, feline, and porcine pro-EGF.

References

  1. Singh, A.B. and R.C. Harris (2005) Cell. Signal. 17:1183.
  2. Wouters, M.A. et al. (2005) Prot. Sci. 14:1091.
  3. Sanderson, M.P. et al. (2006) Growth Factors 24:121.
  4. Gray, A. et al. (1983) Nature 303:722.
  5. Jorissen, R.N. et al. (2003) Exp. Cell Res. 284:31.
  6. Rall, L.B. et al. (1985) Nature 313:228.
  7. Le Gall, S.M. et al. (2004) Regul. Pept. 122:119.
  8. Breyer, J.A. and S. Cohen (1990) J. Biol. Chem. 265:16564.
  9. Parries, G. et al. (1995) J. Biol. Chem. 270:27954.
  10. Le Gall, S.M. et al. (2003) J. Biol. Chem. 278:45255.
  11. Lakshmanan, J. et al. (1990) Biochem. Biophys. Res. Commun. 173:902.
  12. Pasquini, F. et al. (1974) Exp. Cell Res. 86:233.
  13. Pyka, J. et al. (2005) Cancer Res. 65:1343.

Long Name

Epidermal Growth Factor

Alternate Names

HOMG4, URG, Urogastrone

Entrez Gene IDs

1950 (Human); 13645 (Mouse); 25313 (Rat)

Gene Symbol

EGF

UniProt

P01132

Additional EGF Products

Product Documents for Mouse Pro EGF Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Pro EGF Antibody

For research use only

Citations for Mouse Pro EGF Antibody

Customer Reviews for Mouse Pro EGF Antibody (2)

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Customer Images

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  • Mouse Pro EGF Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Brain tissue
    Species: Mouse
    Verified Customer | Posted 10/26/2021
    Mouse Pro EGF Antibody MAB5256
  • Mouse Pro EGF Antibody
    Name: Feng Li
    Application: Immunocytochemistry
    Sample Tested: Mouse kidney
    Species: Mouse
    Verified Customer | Posted 05/17/2017
    Pro-EGF staining in normal C57 kidney tissue (Green). The right image is negative control with only the secondary antibody added.
    Mouse Pro EGF Antibody MAB5256

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Protocols

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