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Mouse/Rat FABP1/L-FABP Antibody

Catalog #: MAB1565
1 Citation Datasheet / COA / SDS
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MAB1565
MAB1565-SP

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Detection of Mouse FABP1/L‑FABP by Western Blot.
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Citations (1)
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Mouse/Rat FABP1/L-FABP Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects rat FABP1/L‑FABP in direct ELISAs and Western blots. In direct ELISAs and Western blots no cross-reactivity with recombinant rat FABP2, recombinant human FABP3, recombinant mouse (rm) FABP4, or rmFABP5 is observed.
Source
Monoclonal Mouse IgG2B Clone # 220119
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant rat FABP1/L‑FABP
Met1-Ile127
Accession # P02692
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
Perfusion fixed frozen sections of rat liver

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse FABP1/L-FABP antibody by Western Blot. View Larger

Detection of Mouse FABP1/L‑FABP by Western Blot. Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Mouse/ Rat FABP1/L-FABP Monoclonal Antibody (Catalog # MAB1565) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FABP1/L-FABP at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Rat FABP1/L-FABP antibody by Western Blot. View Larger

Detection of Rat FABP1/L‑FABP by Western Blot. Western blot shows lysate of rat liver tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Mouse/Rat FABP1/L-FABP Monoclonal Antibody (Catalog # MAB1565) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FABP1/L-FABP at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Mouse FABP1/L-FABP antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse FABP1/L‑FABP by Simple WesternTM. Simple Western lane view shows lysates of mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP1/L‑FABP at approximately 16 kDa (as indicated) using 10 µg/mL of Mouse Anti-Mouse/Rat FABP1/L‑FABP Monoclonal Antibody (Catalog # MAB1565). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP1/L-FABP

FABP1, also known as liver FABP (L-FABP), Z-protein, and squalese- and sterol-carrier protein (SCP), is predominantly expressed in the liver, intestine, kidney and lung. FABP1 binds free fatty acids and their co-enzyme A derivatives and may be involved in intracellular lipid transport.

Long Name
Fatty Acid-Binding Protein 1
Entrez Gene IDs
2168 (Human); 14080 (Mouse); 24360 (Rat)
Alternate Names
FABP1; FABPL; fatty acid binding protein 1, liver; fatty acid-binding protein, liver; LFABP; L-FABP; L-FABPFatty acid-binding protein 1; Liver-type fatty acid-binding protein

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Citation for Mouse/Rat FABP1/L-FABP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Helper-dependent adenovirus-mediated short hairpin RNA expression in the liver activates the interferon response.
    Authors: Witting SR, Brown M, Saxena R, Nabinger S, Morral N
    J. Biol. Chem., 2007-11-19;283(4):2120-8.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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