Mouse Reelin Antibody

Reelin in Mouse Embryo.

Reelin was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Reelin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3820) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Reelin by Western Blot

Expression of UPR genes in control and dcKO brains(A) Total RNA isolated from E16.5 control or dcKO cerebral cortex was subjected to RNA-sequencing (n = 3 brains per group). Heatmaps show gene expressions in each GO term or in ATF6 branch including molecular chaperones in the ER. Color scale bars indicate Z score by row.(B) RT-qPCR. Brains samples from E16.5 control or dcKO cerebral cortex were subjected to RT-qPCR with specific primers for the indicated genes (n = 6–7 brains per group). Expressions levels of molecular chaperones in the ER are downregulated, while those of IRE1-and PERK-downstream genes are upregulated in dcKO brains.(C) Western blotting. Protein was extracted from cerebral cortices of E16.5, and subjected to western blotting with indicated antibodies (n = 3–7 brains per group). Expression of REELIN was reduced in dcKO brain.(D and E) Immunohistochemistry of brain section from E16.5 control and dcKO embryos for p-PERK and SOX2. p-PERK(+) cells are distributed around ventricular zone (D) and majority of them are co-localized with SOX2(+) cells (E) (n = 4 brains per group). Cx, cortex; CPu, caudate putamen. Data are represented as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 by Mann-Whitney U test. Scale bars: 500 μm (D), 50 μm (E). Image collected and cropped by CiteAb from the following open publication (https://linkinghub.elsevier.com/retrieve/pii/S2589004225008302), licensed under a CC-BY license. Not internally tested by R&D Systems.