Mouse Serum Albumin Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-26510

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot, ELISA, Dot Blot, SDS-Page

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Mouse Serum Albumin Primary Antibodies »

Product Specifications

Immunogen

Mouse Serum Albumin Antibody was produced by repeated immunizations with mouse serum albumin. (Uniprot: P07724)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Mouse Serum Albumin antibody was prepared from monospecific antiserum by immunoaffinity chromatography using purified mouse serum albumin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Analysis by SDS-PAGE was used to determine that this preparation is substantially free of aggregates and shows a banding pattern consistent with purified Rabbit IgG.

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Mouse Serum Albumin Antibody - BSA Free

Western Blot: Mouse Serum Albumin Antibody [NBP2-26510]

Western Blot: Mouse Serum Albumin Antibody [NBP2-26510]

Western Blot: Mouse Serum Albumin Antibody [NBP2-26510] - Western Blot of Mouse Serum Albumin antibody. Lane 1: molecular weight. Lane 2: mouse Mouse Serum Albumin. Load: 0.1 ug.Primary antibody: MSA antibody at 1:1000 in 1% BSA-TTBS for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:20,000 for 45 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting 30 min at 4C.Predicted/Observed size: 69 kDa, 72 kDa for MSA. Other band(s): none.
Western Blot: Mouse Serum Albumin Antibody [NBP2-26510]

Western Blot: Mouse Serum Albumin Antibody [NBP2-26510]

Western Blot: Mouse Serum Albumin Antibody [NBP2-26510] - Western Blot: Albumin Antibody [Biotin] [NB600-929] - Lane 1: Human Serum Albumin. Load: 50 ng per lane. Primary antibody: Rabbit anti-Human Serum Albumin Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 66 kDa, 60 kDa for Human Serum Albumin. Image using the Biotin form of this antibody.
SDS-PAGE: Mouse Serum Albumin Antibody [NBP2-26510]

SDS-PAGE: Mouse Serum Albumin Antibody [NBP2-26510]

SDS-Page: Mouse Serum Albumin Antibody [NBP2-26510]
SDS-PAGE: Mouse Serum Albumin Antibody [NBP2-26510]

SDS-PAGE: Mouse Serum Albumin Antibody [NBP2-26510]

SDS-Page: Mouse Serum Albumin Antibody [NBP2-26510] - SDS-PAGE of Mouse Mouse Serum Albumin antibody.Lane 1: mouse serum albumin reduced. Lane 2: molecular weight. Lane 3: mouse serum albumin non-reduced. Load: 1.0 ug.Predicted/Observed size: Analysis by SDS-PAGE shows a banding pattern consistent with purified Rabbit IgG.Other band(s): none.
ELISA: Mouse Serum Albumin Antibody [NBP2-26510]

ELISA: Mouse Serum Albumin Antibody [NBP2-26510]

ELISA: Mouse Serum Albumin Antibody [NBP2-26510] - ELISA results of purified Mouse Mouse Serum Albumin antibody tested against Mouse Mouse Serum Albumin. Each well was coated in duplicate with 1.0 ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.
Mouse Serum Albumin Antibody

Mouse Serum Albumin Antibody

Western Blot of rabbit anti-mouse serum albumin. Lane 1: molecular weight. Lane 2: mouse serum albumin. Load: 0.1 ug. Primary antibody: MSA antibody at 1:1000 in 1% BSA-TTBS for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:20,000 for 45 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting

Applications for Mouse Serum Albumin Antibody - BSA Free

Application
Recommended Usage

ELISA

1:20000-1:100000

Immunohistochemistry

1:500-1:2500

Western Blot

1:2000-1:10000
Application Notes
This product has been tested by ELISA and western blot and is assayed against 1.0 ug of Mouse Serum Albumin in a standard ELISA using Peroxidase conjugated Affinity Purified Goat anti-Rabbit IgG [H&L] MX and TMB as a substrate for 30 minutes at room temperature.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: Mouse Serum Albumin

Anti-Mouse Serum Albumin antibody detects Serum Albumin. Serum albumin is the main protein of plasma. It has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. ALB is the major zinc transporter in plasma; it typically binds about 80% of all plasma zinc.

Alternate Names

Alb1, Alb-1, albumin 1, BCL002

Gene Symbol

ALB

UniProt

P07724 (Mouse)

Additional Mouse Serum Albumin Products

Product Documents for Mouse Serum Albumin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Mouse Serum Albumin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Mouse Serum Albumin Antibody - BSA Free

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Protocols

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FAQs

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