Mouse WISP-1/CCN4 Antibody

R&D Systems | Catalog # AF1680

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all WISP-1/CCN4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse WISP‑1/CCN4
Thr23-Asn367
Accession # O54775

Specificity

Detects mouse WISP‑1/CCN4 in direct ELISAs and Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant human (rh) WISP-1 is observed and less than 1% cross-reactivity with rhCTGF and recombinant mouse NOV is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse WISP-1/CCN4 Antibody

WISP‑1/CCN4 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

WISP‑1/CCN4 in Mouse Embryo.

WISP-1/CCN4 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using 15 µg/mL Sheep Anti-Mouse WISP-1/CCN4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1680) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to developing epithelial cells in duodenum. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of WISP-1/CCN4 by Immunohistochemistry

Detection of WISP-1/CCN4 by Immunohistochemistry

CCN4 deletion reduced AAA formation. Aneurysm grade score—representative images of aortic sections stained with EVG and identified as grade 0–4. ApoE−/−CCN4−/− and ApoE−/−CCN4+/+ mice were exposed to AngII for 28 days and aortic sections stained with EVG (a). The mean aneurysm grade score was calculated from the aortic segment exhibiting the highest degree of aneurysm (most dilated/diseased section per aorta graded) (b). The presence (grey/black bars) and absence (white bars) of vessel wall remodelling on the aorta (presence of fibrous adventitial thickening, as seen in grade 3 example, in any of the sections along the aorta) was noted (c). The number of elastin breaks was quantified from the average of 4 thoracic or 4 abdominal aortic segments (d). Representative images of EVG stained aortae to illustrate vessel wall remodelling (e) and elastin breaks (f), indicated by arrowheads at both low and high power. g Physical parameters of the average thoracic and abdominal aortic sections. CCN4+/+ApoE−/− n = 13 and CCN4−/−ApoE−/− n = 12, * indicates p < 0.05 compared to CCN4 + / + controls. Mann–Whitney test for aneurysm score, elastin breaks and physical parameters, data is presented as mean ± sem (B and D); Fisher’s Exact test for adventitial thickening (C) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34080128), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse WISP-1/CCN4 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (13 d.p.c.)

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse WISP‑1/CCN4 (Catalog # 1680-WS)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: WISP-1/CCN4

Mouse WISP-1 (Wnt-induced secreted protein-1; also named CNN4) is a 40 kDa, secreted, heparin-binding glycoprotein that is a member of the CCN (or CTGF/Cyr61/Nov) cysteine-rich protein family (1‑6). It is synthesized as a 367 amino acid (aa) precursor that contains a series of structural homology modules. Following a 22 aa signal sequence, there is a 68 aa IGFBP-like domain (aa 53‑120), a 66 aa von Willebrand factor type C (VWC) module (aa 121‑186), a 41 aa TSP type I domain (aa 220‑260) and a 75 aa, C-terminal cysteine knot motif (aa 273‑347) (1, 6). The VWC module is associated with protein-protein interaction, the TSP domain binds sulfated glycoconjugates, and the cysteine knot mediates dimerization and receptor binding (4). It is likely that WISP-1 normally circulates as an 80 kDa homodimer (2). Multiple splice forms are reported for human WISP-1, but none for rodent (7‑9). Mature mouse WISP-1 is 98%, 84% and 85% aa identical to rat, canine and human WISP-1, respectively. WISP-1 is expressed by fibroblasts and osteoblasts, and may contribute to fracture healing by promoting bone cell formation (1, 10, 11).

References

  1. Pennica, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:14717.
  2. Tanada, S. et al. (2001) Oncogene 20:5525.
  3. Brigstock, D.R. et al. (2003) J. Clin. Pathol. Mol. Pathol. 56:127.
  4. Desnoyers, L. (2004) Curr. Pharm. Des. 10:3913.
  5. Brigstock, D.R. (2003) J. Endocrinol. 178:169.
  6. Hashimoto, Y. et al. (1998) J. Exp. Med. 187:289.
  7. SwissProt Accession # O54775.
  8. SwissProt Accession # Q99PP0.
  9. SwissProt Accession # O95388
  10. French, D.M. et al. (2004) Am. J. Pathol. 165:855.
  11. Parisi, M.S. et al. (2006) Bone 38:671.

Long Name

Wnt-1 Induced Secreted Protein 1

Alternate Names

CCN4, WISP1

Entrez Gene IDs

8840 (Human); 22402 (Mouse); 102125044 (Cynomolgus Monkey)

Gene Symbol

CCN4

UniProt

O54775

Additional WISP-1/CCN4 Products

Product Documents for Mouse WISP-1/CCN4 Antibody

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Product Specific Notices for Mouse WISP-1/CCN4 Antibody

For research use only

Citations for Mouse WISP-1/CCN4 Antibody

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