MRG-binding protein Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84936

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LFHAMLGHKPVGVNRHFHMICIRDKFSQNIGRQVPSKVIWDHLSTMYDMQALHESEILPFPNPERNFVLPEEIIQEVREGKVMIEEEMKEEMKEDVDPHNGADDVFSSSGSLGKASEKSSKDKEKNSSDLGCKE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MRG-binding protein Antibody - BSA Free

Immunohistochemistry-Paraffin: MRG-binding protein Antibody [NBP1-84936]

Immunohistochemistry-Paraffin: MRG-binding protein Antibody [NBP1-84936]

Immunohistochemistry-Paraffin: MRG-binding protein Antibody [NBP1-84936] - Analysis in human testis and liver tissues using NBP1-84936 antibody. Corresponding MRGBP RNA-seq data are presented for the same tissues.
Western Blot: MRG-binding protein Antibody [NBP1-84936]

Western Blot: MRG-binding protein Antibody [NBP1-84936]

Western Blot: MRG-binding protein Antibody [NBP1-84936] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-352
Immunocytochemistry/ Immunofluorescence: MRG-binding protein Antibody [NBP1-84936]

Immunocytochemistry/ Immunofluorescence: MRG-binding protein Antibody [NBP1-84936]

Immunocytochemistry/Immunofluorescence: MRG-binding protein Antibody [NBP1-84936] - Staining of human cell line U-2 OS shows localization to nucleus.
Immunohistochemistry-Paraffin: MRG-binding protein Antibody [NBP1-84936]

Immunohistochemistry-Paraffin: MRG-binding protein Antibody [NBP1-84936]

Immunohistochemistry-Paraffin: MRG-binding protein Antibody [NBP1-84936] - Staining of human Skeletal muscle shows very weak nuclear positivity in myocytes.
Western Blot: MRG-binding protein Antibody [NBP1-84936]

Western Blot: MRG-binding protein Antibody [NBP1-84936]

Western Blot: MRG-binding protein Antibody [NBP1-84936] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: MRG-binding protein Antibody [NBP1-84936]

Immunohistochemistry-Paraffin: MRG-binding protein Antibody [NBP1-84936]

Immunohistochemistry-Paraffin: MRG-binding protein Antibody [NBP1-84936] - Staining of human Liver shows very weak nuclear positivity in hepatocytes.
Immunohistochemistry-Paraffin: MRG-binding protein Antibody [NBP1-84936]

Immunohistochemistry-Paraffin: MRG-binding protein Antibody [NBP1-84936]

Immunohistochemistry-Paraffin: MRG-binding protein Antibody [NBP1-84936] - Staining of human Pancreas shows very weak nuclear positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: MRG-binding protein Antibody [NBP1-84936]

Immunohistochemistry-Paraffin: MRG-binding protein Antibody [NBP1-84936]

Immunohistochemistry-Paraffin: MRG-binding protein Antibody [NBP1-84936] - Staining of human Testis shows strong nuclear positivity in cells in seminiferous ducts.

Applications for MRG-binding protein Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MRG-binding protein

C20orf20 is a component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and

Alternate Names

chromosome 20 open reading frame 20, Eaf7, FLJ10914, MRG(MORF4-related gene)-binding protein, MRG15BP, MRG-binding protein, MRGBPURCC4

Gene Symbol

MRGBP

Additional MRG-binding protein Products

Product Documents for MRG-binding protein Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MRG-binding protein Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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