MRPL12 Antibody (3B12-1A3) - Azide and BSA Free

Novus Biologicals | Catalog # H00006182-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Immunocytochemistry/ Immunofluorescence, Super-Resolution Microscopy

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3B12-1A3

Format

Azide and BSA Free
View all MRPL12 Primary Antibodies »

Product Specifications

Immunogen

MRPL12 (AAH02344, 1 a.a. ~ 198 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLPAAARPLWGPCLGLRAAAFRLARRQVPCVCAVRHMRSSGHQRCEALAGAPLDNAPKEYPPKIQQLVQDIASLTLLEISDLNELLKKTLKIQDVGLVPMGGVMSGAVPAAAAQEAVEEDIPIAKERTHFTVRLTEAKPVDKVKLIKEIKNYIQGINLVQAKKLVESLPQEIKANVAKAEAEKIKAALEAVGGTVVLE

Specificity

MRPL12 - mitochondrial ribosomal protein L12

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MRPL12 Antibody (3B12-1A3) - Azide and BSA Free

Western Blot: MRPL12 Antibody (3B12-1A3) [H00006182-M01]

Western Blot: MRPL12 Antibody (3B12-1A3) [H00006182-M01]

Western Blot: MRPL12 Antibody (3B12-1A3) [H00006182-M01] - Analysis of MRPL12 expression in transfected 293T cell line by MRPL12 monoclonal antibody (M01), clone 3B12-1A3.Lane 1: MRPL12 transfected lysate(21.3 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: MRPL12 Antibody (3B12-1A3) [H00006182-M01]

Immunocytochemistry/ Immunofluorescence: MRPL12 Antibody (3B12-1A3) [H00006182-M01]

Immunocytochemistry/Immunofluorescence: MRPL12 Antibody (3B12-1A3) [H00006182-M01] - Analysis of monoclonal antibody to MRPL12 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: MRPL12 Antibody (3B12-1A3) [H00006182-M01]

Immunohistochemistry-Paraffin: MRPL12 Antibody (3B12-1A3) [H00006182-M01]

Immunohistochemistry-Paraffin: MRPL12 Antibody (3B12-1A3) [H00006182-M01] - Analysis of monoclonal antibody to MRPL12 on formalin-fixed paraffin-embedded human breast cancer tissue. Antibody concentration 3 ug/ml.
Western Blot: MRPL12 Antibody (3B12-1A3) [H00006182-M01]

Western Blot: MRPL12 Antibody (3B12-1A3) [H00006182-M01]

Western Blot: MRPL12 Antibody (3B12-1A3) [H00006182-M01] - Analysis of MRPL12 over-expressed 293 cell line, cotransfected with MRPL12 Validated Chimera RNAi ( Cat # H00006182-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MRPL12 monoclonal antibody (M01), clone 3B12-1A3 (Cat # H00006182-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: MRPL12 Antibody (3B12-1A3) [H00006182-M01]

Western Blot: MRPL12 Antibody (3B12-1A3) [H00006182-M01]

Western Blot: MRPL12 Antibody (3B12-1A3) [H00006182-M01] - MRPL12 monoclonal antibody (M01), clone 3B12-1A3 Analysis of MRPL12 expression in COLO 320 HSR.
ELISA: MRPL12 Antibody (3B12-1A3) [H00006182-M01]

ELISA: MRPL12 Antibody (3B12-1A3) [H00006182-M01]

ELISA: MRPL12 Antibody (3B12-1A3) [H00006182-M01] - Detection limit for recombinant GST tagged MRPL12 is approximately 0.03ng/ml as a capture antibody.

Applications for MRPL12 Antibody (3B12-1A3) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recominant protein and cell lysate for WB. It has been used for IF, IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MRPL12

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex.

Alternate Names

5c5-2, FLJ60124, L12mt, MGC8610, mitochondrial ribosomal protein L12, MRP-L12, MRP-L31/34, MRPL7, MRPL7/L12, RPML1239S ribosomal protein L12, mitochondrial

Entrez Gene IDs

6182 (Human)

Gene Symbol

MRPL12

OMIM

602375 (Human)

UniProt

AAH02344 (Human)

Additional MRPL12 Products

Product Documents for MRPL12 Antibody (3B12-1A3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MRPL12 Antibody (3B12-1A3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MRPL12 Antibody (3B12-1A3) - Azide and BSA Free

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Protocols

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FAQs

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