MRPL45 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82763

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MRPL45 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GIFDAYVPPEGDARISSLSKEGLIERTERMKKTMASQVSIRRIKDYDANFKIKDFPEKAKDIFIEAHLCLNNSDHD

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MRPL45 Antibody - BSA Free

Western Blot: MRPL45 Antibody [NBP1-82763]

Western Blot: MRPL45 Antibody [NBP1-82763]

Western Blot: MRPL45 Antibody [NBP1-82763] - Analysis using Anti-MRPL45 antibody NBP1-82763 (A) shows similar pattern to independent antibody NBP1-82764 (B).
Immunocytochemistry/ Immunofluorescence: MRPL45 Antibody [NBP1-82763]

Immunocytochemistry/ Immunofluorescence: MRPL45 Antibody [NBP1-82763]

Immunocytochemistry/Immunofluorescence: MRPL45 Antibody [NBP1-82763] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Immunohistochemistry-Paraffin: MRPL45 Antibody [NBP1-82763]

Immunohistochemistry-Paraffin: MRPL45 Antibody [NBP1-82763]

Immunohistochemistry-Paraffin: MRPL45 Antibody [NBP1-82763] - Staining of human small intestine strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: MRPL45 Antibody [NBP1-82763]

Immunohistochemistry-Paraffin: MRPL45 Antibody [NBP1-82763]

Immunohistochemistry-Paraffin: MRPL45 Antibody [NBP1-82763] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: MRPL45 Antibody [NBP1-82763]

Immunohistochemistry-Paraffin: MRPL45 Antibody [NBP1-82763]

Immunohistochemistry-Paraffin: MRPL45 Antibody [NBP1-82763] - Staining of human kidney, liver, prostate and small intestine using Anti-MRPL45 antibody NBP1-82763 (A) shows similar protein distribution across tissues to independent antibody NBP1-82764 (B).
Immunohistochemistry-Paraffin: MRPL45 Antibody [NBP1-82763]

Immunohistochemistry-Paraffin: MRPL45 Antibody [NBP1-82763]

Immunohistochemistry-Paraffin: MRPL45 Antibody [NBP1-82763] - Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: MRPL45 Antibody [NBP1-82763]

Immunohistochemistry-Paraffin: MRPL45 Antibody [NBP1-82763]

Immunohistochemistry-Paraffin: MRPL45 Antibody [NBP1-82763] - Staining of human prostate shows moderate granular cytoplasmic positivity in glandular cells.

Applications for MRPL45 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MRPL45

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within themitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. Theyhave an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed.Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA.Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes inbiochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein.Pseudogenes corresponding to this gene are found on chromosomes 2p and 17q. (provided by RefSeq)

Alternate Names

L45mt, MGC11321, mitochondrial ribosomal protein L45, MRP-L45,39S ribosomal protein L45, mitochondrial

Gene Symbol

MRPL45

Additional MRPL45 Products

Product Documents for MRPL45 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MRPL45 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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