MSH6 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-33151

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Zebrafish

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MSH6 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human MSH6. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

135 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MSH6 Antibody - BSA Free

Knockout Validated: MSH6 Antibody [NBP1-33151]

Western Blot: MSH6 Antibody [NBP1-33151]

Western Blot: MSH6 Antibody [NBP1-33151] - Wild-type (WT) and MSH6 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MSH6 antibody [C1C2], Internal. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: MSH6 Antibody [NBP1-33151]

Western Blot: MSH6 Antibody [NBP1-33151]

Western Blot: MSH6 Antibody [NBP1-33151] - Sample (30 ug of whole cell lysate) A: Hep G2 5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: MSH6 Antibody [NBP1-33151]

Immunocytochemistry/ Immunofluorescence: MSH6 Antibody [NBP1-33151]

Immunocytochemistry/Immunofluorescence: MSH6 Antibody [NBP1-33151] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: MSH6 Antibody [NBP1-33151]

Immunohistochemistry-Paraffin: MSH6 Antibody [NBP1-33151]

Immunohistochemistry-Paraffin: MSH6 Antibody [NBP1-33151] - Paraffin-embedded NCIN87 Xenograft, using antibody at 1:100 dilution.
Western Blot: MSH6 Antibody [NBP1-33151]

Western Blot: MSH6 Antibody [NBP1-33151]

Western Blot: MSH6 Antibody [NBP1-33151] - A. 30 ug Neuro2A whole cell lysate/extract, B. 30 ug GL261 whole cell lysate/extract 5% SDS-PAGE gel, antibody dilution 1:1000.
Immunoprecipitation: MSH6 Antibody [NBP1-33151]

Immunoprecipitation: MSH6 Antibody [NBP1-33151]

Immunoprecipitation: MSH6 Antibody [NBP1-33151] - MSH6 antibody immunoprecipitates MSH6 protein in IP experiments. IP samples: 293T whole cell extract A. 40 ug 293T whole cell extract. B. Control with 4 ug of preimmune Rabbit IgG. C. Immunoprecipitation of MSH6 protein by 4 ug MSH6 antibody. 5 % SDS-PAGE. The immunoprecipitated MSH6 protein was detected by MSH6 antibody diluted at 1:1000.

Applications for MSH6 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000
Application Notes
EMSA Assay dependent.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MSH6

MSH6 is a mismatch-repair protein. It plays a role in tumor suppression. Genomic mutations in MSH6 predispose to the hereditary non-polyposis colorectal cancer (HNPCC) syndrome.

Long Name

mutS homolog 6

Alternate Names

GTBP, GTMBP, HNPCC5, HSAP, LYNCH5, MMRCS3, MSH-6, p160

Gene Symbol

MSH6

Additional MSH6 Products

Product Documents for MSH6 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MSH6 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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