MST1/STK4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82865

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MST1/STK4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IEHDDTLPSQLGTMVINAEDEEEEGTMKRRDETMQPAKPSFLEYFEQKEKENQINSFGKSVPGPLKNSSDWKIPQDGDYEF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit MST1/STK4 Antibody - BSA Free (NBP1-82865) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-MST1/STK4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for MST1/STK4 Antibody - BSA Free

Western Blot: STK4 Antibody [NBP1-82865]

Western Blot: STK4 Antibody [NBP1-82865]

Western Blot: STK4 Antibody [NBP1-82865] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: STK4 Antibody [NBP1-82865]

Immunocytochemistry/ Immunofluorescence: STK4 Antibody [NBP1-82865]

Immunocytochemistry/Immunofluorescence: STK4 Antibody [NBP1-82865] - Staining of human cell line CACO-2 shows localization to nucleoplasm, nuclear bodies & cytosol.
Immunohistochemistry-Paraffin: STK4 Antibody [NBP1-82865]

Immunohistochemistry-Paraffin: STK4 Antibody [NBP1-82865]

Immunohistochemistry-Paraffin: STK4 Antibody [NBP1-82865] - Staining of human lymph node shows strong cytoplasmic positivity in cells outside reaction centra.
Western Blot: STK4 Antibody [NBP1-82865]

Western Blot: STK4 Antibody [NBP1-82865]

Western Blot: STK4 Antibody [NBP1-82865] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Applications for MST1/STK4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MST1/STK4

STK4 is encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process.

Long Name

Mammalian STE20-like Protein Kinase 1/Serine/Threonine Protein Kinase 4

Alternate Names

KRS2, STK4, YSK3

Gene Symbol

STK4

Additional MST1/STK4 Products

Product Documents for MST1/STK4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MST1/STK4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MST1/STK4 Antibody - BSA Free

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Protocols

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