MT-CO2 Antibody (6H0N5)

Novus Biologicals | Catalog # NBP3-16283

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6H0N5 expressed in HEK293
View all MT-CO2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 128-227 of human MT-CO2 (P00403). LEPGDLRLLDVDNRVVLPIEAPIRMMITSQDVLHSWAVPTLGLKTDAIPGRLNQTTFTATRPGVYYGQCSEICGANHSFMPIVLELIPLKIFEMGPVFTL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MT-CO2 Antibody (6H0N5)

Western Blot: MT-CO2 Antibody (6H0N5) [NBP3-16283]

Western Blot: MT-CO2 Antibody (6H0N5) [NBP3-16283]

Western Blot: MT-CO2 Antibody (6H0N5) [NBP3-16283] - Western blot analysis of extracts of various cell lines, using MT-CO2 Rabbit mAb (NBP3-16283) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
MT-CO2 Antibody (6H0N5)

Immunohistochemistry-Paraffin: MT-CO2 Antibody (6H0N5) [NBP3-16283] -

Immunohistochemistry-Paraffin: MT-CO2 Antibody (6H0N5) [NBP3-16283] - Human liver tissue using MTCO2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
MT-CO2 Antibody (6H0N5)

Immunohistochemistry- MT-CO2 Antibody (6H0N5) [NBP3-16283] -

Immunohistochemistry- MT-CO2 Antibody (6H0N5) [NBP3-16283] -Confocal imaging of HeLa cells using MTCO2 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
MT-CO2 Antibody (6H0N5)

Immunohistochemistry: MT-CO2 Antibody (6H0N5) [NBP3-16283] -

Immunohistochemistry: MT-CO2 Antibody (6H0N5) [NBP3-16283] - Immunohistochemistry analysis of paraffin-embedded Human brain tissue using MT-CO2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
MT-CO2 Antibody (6H0N5)

Immunocytochemistry/ Immunofluorescence: MT-CO2 Antibody (6H0N5) [NBP3-16283] -

Immunocytochemistry/ Immunofluorescence: MT-CO2 Antibody (6H0N5) [NBP3-16283] - Confocal imaging of HeLa cells using MT-CO2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
MT-CO2 Antibody (6H0N5)

Immunocytochemistry/ Immunofluorescence: MT-CO2 Antibody (6H0N5) [MT-CO2] -

Immunocytochemistry/ Immunofluorescence: MT-CO2 Antibody (6H0N5) [MT-CO2] - Confocal imaging of paraffin-embedded Human liver tissue using MT-CO2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Applications for MT-CO2 Antibody (6H0N5)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL

Immunocytochemistry/ Immunofluorescence

1:200 - 1:800

Immunohistochemistry

1:200 - 1:800

Immunohistochemistry-Paraffin

1:200 - 1:800

Western Blot

1:1000 - 1:6000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MT-CO2

Alternate Names

COII, COX2, COXII, Cytochrome C Oxidase II, Cytochrome C Oxidase Polypeptide II, Cytochrome C Oxidase Subunit II, EC 1.9.3.1, Mitochondrially Encoded Cytochrome C Oxidase II, MTCO2

Gene Symbol

COX2

Additional MT-CO2 Products

Product Documents for MT-CO2 Antibody (6H0N5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MT-CO2 Antibody (6H0N5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MT-CO2 Antibody (6H0N5)

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Protocols

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FAQs

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