MTIF2 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-95177

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all MTIF2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 428-727 of human MTIF2 (NP_002444.2). GEEILEVESEPRAREVVDWRKYEQEQEKGQEDLKIIEEKRKEHKEAHQKAREKYGHLLWKKRSILRFLERKEQIPLKPKEKRERDSNVLSVIIKGDVDGSVEAILNIIDTYDASHECELELVHFGVGDVSANDVNLAETFDGVIYGFNVNAGNVIQQSAAKKGVKIKLHKIIYRLVEDLQEELSSRLPCAVEEHPVGEASILATFSVTEGKKKVPVAGCRVQKGQLEKQKKFKLTRNGHVIWKGSLTSLKHHKDDISIVKTGMDCGLSLDEDNMEFQVGDRIVCYEEKQIQAKTSWDPGF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MTIF2 Antibody - Azide and BSA Free

Western Blot: MTIF2 AntibodyAzide and BSA Free [NBP2-95177]

Western Blot: MTIF2 AntibodyAzide and BSA Free [NBP2-95177]

Western Blot: MTIF2 Antibody [NBP2-95177] - Analysis of extracts of various cell lines, using MTIF2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.
Immunohistochemistry-Paraffin: MTIF2 Antibody - Azide and BSA Free [NBP2-95177]

Immunohistochemistry-Paraffin: MTIF2 Antibody - Azide and BSA Free [NBP2-95177]

Immunohistochemistry-Paraffin: MTIF2 Antibody [NBP2-95177] - Paraffin-embedded human lung cancer using MTIF2.
Immunohistochemistry-Paraffin: MTIF2 Antibody - Azide and BSA Free [NBP2-95177]

Immunohistochemistry-Paraffin: MTIF2 Antibody - Azide and BSA Free [NBP2-95177]

Immunohistochemistry-Paraffin: MTIF2 Antibody [NBP2-95177] - Paraffin-embedded human colon carcinoma using MTIF2.
MTIF2 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: MTIF2 Antibody - Azide and BSA Free [MTIF2] -

Immunocytochemistry/ Immunofluorescence: MTIF2 Antibody - Azide and BSA Free [MTIF2] - Immunofluorescence analysis of C6 cells using MTIF2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
MTIF2 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: MTIF2 Antibody - Azide and BSA Free [MTIF2] -

Immunocytochemistry/ Immunofluorescence: MTIF2 Antibody - Azide and BSA Free [MTIF2] - Immunofluorescence analysis of L929 cells using MTIF2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for MTIF2 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:50-1:100

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MTIF2

During the initiation of protein biosynthesis, initiation factor-2 (IF-2) promotes the binding of the initiator tRNA to the small subunit of the ribosome in a GTP-dependent manner. Prokaryotic IF-2 is a single polypeptide, while eukaryotic cytoplasmic IF-2 (eIF-2) is a trimeric protein. Bovine liver mitochondria contain IF-2(mt), an 85-kD monomeric protein that is equivalent to prokaryotic IF-2. The predicted 727-amino acid human protein contains a 29-amino acid presequence. Human IF-2(mt) shares 32 to 38% amino acid sequence identity with yeast IF-2(mt) and several prokaryotic IF-2s, with the greatest degree of conservation in the G domains of the proteins. Two transcript variants encoding the same protein have been found for this gene. (provided by RefSeq)

Alternate Names

IF-2(Mt), mitochondrial, mitochondrial translational initiation factor 2

Gene Symbol

MTIF2

Additional MTIF2 Products

Product Documents for MTIF2 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MTIF2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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