MURF3 Antibody

Novus Biologicals | Catalog # NB100-1197

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MURF3 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-GSAGPEEERPDGP corresponding to C-Terminus according to NP_115935.3, NP_912730.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MURF3 Antibody

Western Blot: MURF3 Antibody [NB100-1197]

Western Blot: MURF3 Antibody [NB100-1197]

Western Blot: MURF3 Antibody [NB100-1197] - (1ug/ml) of human muscle lysate (RIPA buffer, 35ug total
Immunohistochemistry-Paraffin: MURF3 Antibody [NB100-1197]

Immunohistochemistry-Paraffin: MURF3 Antibody [NB100-1197]

Immunohistochemistry-Paraffin: MURF3 Antibody [NB100-1197] - Staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for MURF3 Antibody

Application
Recommended Usage

Immunohistochemistry

3.75 ug/ml

Immunohistochemistry-Paraffin

3.75 ug/ml

Peptide ELISA

Detection limit 1:8000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Experiments gave bands at approx. 45 kDa band and 38 kDa band in human skeletal muscle lysate after 1 ug/ml antibody staining. These bands correspond to the reported isoforms with calculated MWs of 43.1 kDa band according to NP_115935, and 38.6 kDa band according to NP_912730.
IHC: Paraffin embedded Human Skeletal Muscle.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MURF3

The protein encoded by this gene contains a RING finger motif and is highly similar to the ring finger proteins RNF28/MURF1 and RNF29/MURF2. In vitro studies demonstrated that this protein, RNF28, and RNF29 form heterodimers, which may be important for the regulation of titin kinase and microtubule-dependent signal pathways in striated muscles. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Alternate Names

MuRF, MURF3, MURF-3, MURFtripartite motif-containing protein 54, Muscle-specific RING finger protein, Muscle-specific RING finger protein 3, RING finger protein 30muscle-specific RING-finger protein 3, RNF30, tripartite motif containing 54, tripartite motif-containing 54

Entrez Gene IDs

57159 (Human)

Gene Symbol

TRIM54

UniProt

NP_115935. (Human)

Additional MURF3 Products

Product Documents for MURF3 Antibody

Certificate of Analysis

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Product Specific Notices for MURF3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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