Mus81 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-95212

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human MUS81 (NP_079404.3). MAAPVRLGRKRPLPACPNPLFVRWLTEWRDEATRSRRRTRFVFQKALRSLRRYPLPLRSGKEAKILQHFGDGLCRMLDERLQRHRTSGGDHAPDSPSGENSPAPQGRLAEVQDSSMPVPAQPKAGGSGSYWPARHSGARVILLVLYREHLNPNGHHFLTKEELLQRCAQKSPRVAPGSARPWPALRSLLHRNLVLRTHQPARYSLTPEGLELAQKLAESEGLSLLNVGIGPKEPPGEETAVPGAASAELASEAGVQQQPLELRPGEYRVLLCVDIGETRGGGHRPELLRELQRLHVTHTVRKLHVGDFVWVAQETNPRDPANPGELVLDHIVERKRLDDLCSSIIDGRFR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Mus81 Antibody - BSA Free

Western Blot: Mus81 AntibodyBSA Free [NBP2-95212]

Western Blot: Mus81 AntibodyBSA Free [NBP2-95212]

Western Blot: Mus81 Antibody [NBP2-95212] - Analysis of extracts of various cell lines, using Mus81 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
Immunohistochemistry-Paraffin: Mus81 Antibody - BSA Free [NBP2-95212]

Immunohistochemistry-Paraffin: Mus81 Antibody - BSA Free [NBP2-95212]

Immunohistochemistry-Paraffin: Mus81 Antibody [NBP2-95212] - Rat brain using MUS81 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: Mus81 Antibody - BSA Free [NBP2-95212]

Immunohistochemistry-Paraffin: Mus81 Antibody - BSA Free [NBP2-95212]

Immunohistochemistry-Paraffin: Mus81 Antibody [NBP2-95212] - Mouse brain using MUS81 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for Mus81 Antibody - BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:50-1:100

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Mus81

Mus81 and EME1 constitute a heterodimeric endonuclease that has been implicated in mitotic and meiotic recombination in eukaryotes. Reduction or loss of a protein called Mus81 can led to the development of lymphomas and other tumours in mice. Mus81 is a protein long thought to be involved in repairing DNA that is damaged during normal metabolic processes.

Alternate Names

crossover junction endonuclease MUS81, EC 3.1.22, EC 3.1.22.-, FLJ21012, FLJ44872, MUS81 endonuclease homolog (S. cerevisiae), MUS81 endonuclease homolog (yeast), SLX3, SLX3 structure-specific endonuclease subunit homolog

Gene Symbol

MUS81

Additional Mus81 Products

Product Documents for Mus81 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Mus81 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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