![Western Blot: NCKIPSD Antibody [NBP1-42664]](https://resources.rndsystems.com/images/products/NCKIPSD-Antibody-Western-Blot-NBP1-42664-img0004.jpg "Western Blot: NCKIPSD Antibody [NBP1-42664]")
Loading...
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Loading...
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 672 and 722 of human SH3 protein interacting with Nck, 90 kDa using the numbering given in entry NP_057537.1 (GeneID 51517).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NCKIPSD Antibody
Western Blot: NCKIPSD Antibody [NBP1-42664]
Western Blot: NCKIPSD Antibody [NBP1-42664] - Detection of Human SPIN90 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SPIN90 antibody NBP1-42664 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunohistochemistry: NCKIPSD Antibody [NBP1-42664]](https://resources.rndsystems.com/images/products/NCKIPSD-Antibody-Immunohistochemistry-NBP1-42664-img0002.jpg "Immunohistochemistry: NCKIPSD Antibody [NBP1-42664]")
Immunohistochemistry: NCKIPSD Antibody [NBP1-42664]
Immunohistochemistry: NCKIPSD Antibody [NBP1-42664] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- SPIN90 used at a dilution of 1:500 (0.4ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
NCKIPSD Antibody [NBP1-42664] - Detection of human SPIN90 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SPIN90 antibody NBP1-42664 (lot NBP1-42664-2) used for IP at 6 ug per reaction. SPIN90 was also immunoprecipitated by a previous lot of this antibody NBP1-42664-1. For blotting immunoprecipitated SPIN90, NBP1-42664 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Applications for NCKIPSD Antibody
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: NCKIPSD
Alternate Names
54 kDa vimentin-interacting protein, 90 kDa SH3 protein interacting with Nck, AF3p21, AF3P21DIP-1, dia interacting protein, Dia-interacting protein 1, diaphanous protein interacting protein, Diaphanous protein-interacting protein, DIP, DIP1, MGC23891, NCK interacting protein with SH3 domain, ORF1, SPIN90VIP54, WASLBP, WASP-interacting SH3-domain protein, WISHSH3 adapter protein SPIN90, Wisk90 kDa
Entrez Gene IDs
51517 (Human)
Gene Symbol
NCKIPSD
Additional NCKIPSD Products
Product Documents for NCKIPSD Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NCKIPSD Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for NCKIPSD Antibody
There are currently no reviews for this product. Be the first to review NCKIPSD Antibody and earn rewards!
Have you used NCKIPSD Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...