NDRG1 Antibody (4C8) - BSA Free

Immunohistochemistry: NDRG1 Antibody (4C8) [NBP3-26118] -

Immunohistochemistry: NDRG1 Antibody (4C8) [NBP3-26118] - Image of NDRG1 Antibody (4C8) diluted at 1:71.6666666666667 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot: NDRG1 Antibody (4C8) [NBP3-26118] -

Western Blot: NDRG1 Antibody (4C8) [NBP3-26118] - Positive Western Blot detected in: Hela whole cell lysate, 293T whole cell lysate, PC3 whole cell lysate, U87 whole cell lysate, Mouse kidney tissue.
All lanes: NDRG1 Antibody at 0.7ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 43, 36, 34 KDa
Observed band size: 43 KDa

Immunocytochemistry/Immunofluorescence: NDRG1 Antibody (4C8) [NBP3-26118] -

Immunocytochemistry/Immunofluorescence: NDRG1 Antibody (4C8) [NBP3-26118] - Staining of Hela cells with NDRG1 Antibody (4C8) at 1:23, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).